Microscope lab lecture

background will be "white" light and the specimen will be dark

brightfieqld microscope

skeleton of microscope that supports weight

framework

carrying handle (also called neck)

arm

bottom of microscope

base

top of microscope

head

light source

lamp

adjustable disc with a hole in the center

diaphragm (iris)

first of 3 lens systems in your microscope

condenser lens

the condenser lens collects light from ___________ and focuses it into a tight beam

illuminator

slide supporting platform

stage

clam your slide

stage clip

allows you to change slide orientation

stage controls

connected to head via a rebolving nose piece

objective elnses

scanning lens

4x

low power lens

10x

high dry lens

40x

oil immersion lens

100x

the lens you look through

occular lens

the occular lens is ________ or _______

monocular; binocular

the occular lens magnifies____

10x

oxular lens magnification x objective lens magnification

magnificaition

used for focusing your specimen

focus adjustment knobs

the focus adjustment knobs akter the distance between the stage and objective used which is called the ______________

working distance

you use the coarse adjustment knobs are used with __ x and __x

4; 10

you use the fine adjustment knobs with __x and __x

40; 100

what are the 2 goals of microscopy?

magnification and resolution

making things appear larger

maginificiation

clarity of image

resolution

resolution is also the abikity to distinguish 2 adjacent objects as ___________

separate

the minimum distance required between 2 adjacent objects to view them as clear and sperate

resolving power

RP=

0.61 LAMBDA

wavelength of light

LAMBDA

LAMBDA=

500nm

N.A.=

numerical aperture

what is the N.A for 4x

0.1

what is the N.A. for 10x

0.25

what is the N.A. for 40x

0.65

what is the N.A for 100x

1.25

what are the 4factors that provide the best resolution?

1. use the shortest wavelength of light

2. raise condenser lens fully

3. adjust the diaphragm accordingly

4. use oil and the 100x objective lens

the ability to maintain the centerdeness of your image when switching objective lens

parcentric

the ability to maintain the focus of your specimen when switching objective lens

parfocal