LAB - ClinChem Week2 Day1

Universal Precaution.

Treat all human blood samples and any body fluids as infections

Elimination

Hierarchy of Control: ___

• Physically remove the hazard

Substitution

Hierarchy of Control: ___

• Replace the hazard

Engineering Controls

Hierarchy of Control: ___

• Isolate people from hazards

Administrative Controls

Hierarchy of Control: ___

• Change the way people work

PPE

Hierarchy of Control: ___

• Protect the worker with Personal Protective Equipment

Hand washing

What is the most important means of preventing the spread of infection

20 seconds

How long should handwashing be?

Downward

When rinsing your hands during handwashing, what direction should your hands be when rinsing?

Donning

Doffing

Class 2 A1

Biosafety Cabinet: 70% is recirculated, 30% is exhausted INTO the room

Class 2 A2

Biosafety Cabinet: 70% is recirculated, 30% is exhausted INTO the room with a higher intake of air velocity

Class 2 B1

Biosafety Cabinet: 30% Is recirculated, 70% is exhausted to the OUTSIDE of the building

Class 2 B2

Biosafety Cabinet: 100% is exhausted to the outside of the building

5.25% NaOCl or 10% Chlorine Bleach

A decontaminating solution uses ___ or ___

1:10

Decontaminating solution has the dilution of ___ (solute to water)

disposable towels

Before Decontamination: Absorb the blood with ___. Remove as much liquid blood or serum as much as possible.

methyl alcohol

Decontaminate non-disposable equipment by soaking overnight in a diluted bleach solution and rinsing with ___ and water before use

<400 nm

Radiation Biohazard: Ultraviolet frequency

400 - 700 nm

Radiation Biohazard: Visible spectrum frequency

>700 nm

Radiation Biohazard: Infrared frequency

3um - 3mm

Radiation Biohazard: Microwaves frequency

>1cm

Radiation Biohazard: Low frequency frequency

Fume hood

Used for materials that give off harmful vapors; provides personnel protection only

Safety Data Sheet

Formerly MSDS, a major source of information about chemicals written by manufacturers; revised format consists of 16 selections

Water-soluble

Type of chemical waste that has to be flushed down the drain

Flammable/explosive

Type of chemical waste that has to be collected in approved containers or be incinerated

Solid

Type of chemical waste that has to go into a landfill

Biohazard Symbol

Biohazard waste must be placed within a container with a ___

To contain

holds but does NOT deliver the exact volume

To deliver

delivers the exact volume it hold

Blow out

characterized by etched ring near the mouth

Self-draining

allowed to drain by gravity

Air displacement

The piston does NOT come in contact with the fluid

Positive displacement

Operates like hypodermic syringe; No air cushion; constant aspiration force; for high density or very viscous samples

6 months

Calibration of pipettes have a frequency of ___

Gravimetric method

Calibration of pipettes: ___

• weigh the distilled water delivered. Most accurate

Spectrophotometric method

Calibration of pipettes: ___

• absorbance of colored solution delivered