Lab 11: Trematodes

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  • Schistosoma japonicum

  • Schistosoma mansoni

  • Schistosoma haematobium

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153 Terms

1
  • Schistosoma japonicum

  • Schistosoma mansoni

  • Schistosoma haematobium

Blood flukes

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2

Schistosomiasis or Bilharziasis

Schistosoma spp: Disease

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3

IH: Freshwater snail

Schistosoma spp: Intermediate host

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4

Mammalian host

Schistosoma spp: Definitive host

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5

Skin penetration of cercariae

Schistosoma spp: MOT

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Miracidium

Schistosoma spp: Infective stage to snail

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7

Forked tail cercariae

Schistosoma spp: Infective stage to man

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8

Embryonated egg

Schistosoma spp: Diagnostic stage

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9

230

Schistosoma spp:

Total no of cercariae shed in whole infection: Single infection

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10

280

Schistosoma spp:

Total no of cercariae shed in whole infection: Multiple infection

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11

Manson’s Blood fluke

Schistosoma mansoni common name

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12

Biomphalaria

Schistosoma mansoni IH

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13

Vesical blood fluke

Schistosoma haematobium common name

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14

Bulinus

Schistosoma haematobium IH

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15

Oriental Blood fluke

Schistosoma japonicum common name

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16

Oncomelania

Schistosoma japonicum IH

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17
  • Veins of colon

  • Stool, Rectal biopsy

Schistosoma mansoni: Habitat & Specimen

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  • Veins of urinary bladder

  • Urine

Schistosoma haematobiumi: Habitat & Specimen

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  • Veins of small intestine

  • Stool, Rectal biopsy

Schistosoma japonicum: Habitat & Specimen

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Schistosoma japonicum, egg

  • Ovoid, round, pear shaped

  • Pale yellow

  • Thin shell

  • Small lateral spine

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21

Schistosoma japonicum, egg

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Schistosoma japonicum, egg

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Schistosoma mansoni, egg

  • Oblong

  • Large lateral spine

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24

Schistosoma mansoni, egg

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  • Oblong

  • Large terminal spine

Schistosoma haematobium, egg

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Schistosoma haematobium, egg

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500-2000 immature eggs

Schistosoma japonicum, adult

Adult female deposits ________________/ day

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28

gynecophoral canal

[S. japonicum]

Adult male has _____________ that receives the adult female in copulation

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29

Swimmer’s itch

dermatitis with pruritis; localized reaction to the cercarial penetration

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30
  • Swimmer’s itch

  • Katayama fever/ Snail Fever

  • Cor pulmonale

  • Distended belly

symptoms of schistosomiasis

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31

Katayama fever/ Snail Fever

hypersensitivity reaction to the schistosomula migration

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32

Cor pulmonale

obstruction of the pulmonary vasculature

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33

recovery of eggs in stool or rectal imprint/biopsy spx.

Lab diagnosis for S. japonicum, S. mansoni

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34

eggs are recovered from urine spx.

Lab diagnosis for S. haematobium

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35
  • Kato Katz Technique - most suitable for egg quantification

  • MIFT

  • Immunodiagnostic techniques Intradermal test, ELISA, COPT, Indirect hemagglutination

Lab diagnosis for Schistosomiasis

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36

Paragonimiasis, Pulmonary distomiasis

Paragonimus westermani: Disease

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37

Ingestion of undercooked/raw crabs infected with metacercariae

Paragonimus westermani: MOT

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38

Lung

Paragonimus westermani: Habitat

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39

Antemelania asperata, Antemelania dactylus (snails)

Paragonimus westermani: 1st IH

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Sundathelphusa philippina (mountain

Paragonimus westermani: 2nd IH

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Human

Paragonimus westermani: DH

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42

Unembryonated egg

Paragonimus westermani: Diagnostic stage

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Metacercariae

Paragonimus westermani: Infective stage

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44

Paragonimus westermani, adult

  • Reddish brown

  • Coffee bean

  • Rounded anteriorly

  • Slightly tapered posteriorly

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45

Paragonimus westermani, adult

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46

Paragonimus westermani, adult

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Paragonimus westermani, egg

  • Oval

  • Yellowish brown

  • Flattened, prominent operculum

  • Thickened abopercular portion

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48

Paragonimus westermani, egg

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49

Paragonimus westermani, egg

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50
  • Pulmonary Distomiasis /Paragonimiasis

  • Cerebral paragonimiasis

Paragonimus westermani: Clinical manifestation

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51

Pulmonary Distomiasis/Paragonimiasis

mimics Pulmonary tuberculosis symptoms

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52

Cerebral paragonimiasis

migration of immature P. westermani leading to serious neurologic condition

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53

Recovery of eggs from sputum/feces (when swallowed)

Paragonimus westermani: Lab diagnosis

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54

T

[Paragonimus westermani]

T/F: If initial findings in sputum are negative, repeat examination up to three times on different days provides best sensitivity for microscopic analysis

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55
  • CT scan, MRI - characteristic findings of “grape cluster” app (posterior part of the)

  • Skull Radiograph - soap bubble calcifications

Lab diagnosis for Cerebral paragonimiasis

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56
  • Fasciolopsis buski

  • Echinostoma ilocanum

  • Artyfechinostomum malayanum

Intestinal flukes

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57

Fasciolopsis buski

Common name: Giant Intestinal fluke

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Ingestion of encysted metacercariae from aquatic plants

Fasciolopsis buski: MOT

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Duodenum

Fasciolopsis buski: Habitat

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60

Segmentina or Hippeutis (

Fasciolopsis buski: 1st IH

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Water caltrop, Water chestnut, Morning glory (kangkong), Lotus

Fasciolopsis buski: 2nd IH

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Humans, pigs

Fasciolopsis buski: Definitive host

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Unembryonated egg

Fasciolopsis buski: Diagnostic stage

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Metacercariae

Fasciolopsis buski: Infective stage

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Fasciolopsis buski, adult

  • Large, oval

  • Unbranched intestinal ceca

  • does not have a cephalic cone

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Fasciolopsis buski, adult

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Fasciolopsis buski, adult

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Fasciolopsis buski, egg

  • Large

  • Operculated

  • Unembryonated when first passed

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Detection of parasite eggs in stool

Fasciolopsis buski: Lab diagnosis

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70
  • Traumatic - inflammation and ulceration

  • Obstructive - heavy infection

  • Toxic - absorption of worm metabolites by the host

Fasciolopsis buski: Clin. manifestation

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71

Echinostoma ilocanum

Garisson’s fluke

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72

Ingestion of metacercariae encysted in snails

Echinostoma ilocanum: MOT

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Duodenum

Echinostoma ilocanum: Habitat

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74

Gyralus, Hippeutis (Snails)

Echinostoma ilocanum: 1st IH

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Pila luzonica and Vivipara angularis

Echinostoma ilocanum: 2nd IH

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Miracidium

Echinostoma ilocanum: IS to 1st IH

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Cercariae

Echinostoma ilocanum: IS to 2nd IH

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Metacercariae

Echinostoma ilocanum: IS to DH

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79

Humans

Echinostoma ilocanum: DH

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80

Unembryonated eggs

Echinostoma ilocanum: DS

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Echinostoma ilocanum, adult

  • Reddish gray

  • Tapered posterior end with 49-51 collar spines surrounding the oral sucker

  • Simple intestinal ceca

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Echinostoma ilocanum, adult

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Echinostoma ilocanum, adult

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Echinostoma ilocanum, adult

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Echinostoma ilocanum, egg

  • Straw colored

  • Ovoid

  • Operculated

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Echinostoma ilocanum, egg

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Echinostoma ilocanum, egg

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Detection of parasite eggs in stool

Echinostoma ilocanum: Lab diagnosis

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89

Ingestion of metacercariae encysted in fish

Heterophyid flukes: MOT

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90

Small Intestine

Heterophyid flukes: Habitat

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91

Snail

Heterophyid flukes: 1st IH

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92

Freshwater, Brackish Water Fish

Heterophyid flukes: 2nd IH

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Cercariae

Heterophyid flukes: IS to 2nd IH

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Humans, other fresh eating mammals

Heterophyid flukes: DH

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95

Embryonated egg

Heterophyid flukes: DS

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96

base of the fin

[Heterophyid flukes]

Metacercariae are frequently found in the muscles at the ___________

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97

Heterophyes heterophyes

Von Siebold’s fluke

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98

Heterophyes heterophyes, egg

  • Light brown

  • With developed miracidium

  • Thick shell

  • Discrete shoulder

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99

Metagonimus yokogawai

Smallest human fluke

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100

Metagonimus yokogawai, egg

  • Light brown

  • With developed miracidium

  • Thin shell

  • Discrete shoulder

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