MICB 212 Bacteriophage

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6 Terms

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bacteriophage

found everywhere in all natural populations of bacteria

2
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bacteriophage structure

head (capsid)

tail

tail fibers or spikes

base plate

3
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small DNA genomes

< 10 kb

mostly ssDNA

4
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large DNA genomes

medium to large DNA genome (30-200 kb)

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bacteriophage lytic cycle

  1. attach to surface of bacterial cell

  2. inject DNA

  3. gene expression

  4. replicate genome

  5. transcribe & translate genes

  6. assemble phage genomes

  7. encode lysis proteins

  8. lyse bacterial cell

  9. release progeny phages

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bacteriophage lysogenic cycle

  1. cells metabolically sluggish (not much nutrients)

  2. bacteriophage attaches to surface of bacterial cell

  3. establishes itself as prophage

  4. hides as prophage until cell conditions get better

  5. re-enters lytic cycle