W - BIOL200 - 4.1-4.2 GENES AND MRNA, GENOME & CHROMOSOMAL STRUCTURE

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31 Terms

1
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Q: What are the 3 basic elements of a gene?

A: Promoter, coding region, terminator.

2
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Q: What does the promoter do?

A: It’s a DNA region before a gene where RNA polymerase binds and starts transcription.

3
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Q: What does the coding region do?

A: It codes for an RNA molecule (mRNA, rRNA, tRNA).

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Q: What does the terminator do?

A: It signals transcription to stop.

5
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Q: What is the template strand?

A: DNA strand that is transcribed.

6
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Q: What is the coding strand?

A: The complementary strand to the template; same sequence as mRNA except T vs. U.

7
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Q: How is DNA read for transcription?

A: 3′ to 5′ direction.

8
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Q: How is RNA synthesized?

A: 5′ to 3′ direction.

9
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Q: What does the 5′ UTR contain?

A: Ribosome binding site.

10
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Q: What marks where translation begins?

A: Start codon.

11
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Q: What marks where translation stops?

A: Stop codon.

12
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Q: What is polycistronic mRNA?

A: One mRNA strand encodes multiple proteins.

13
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Q: In mRNA synthesis, which strand is used as template?

A: The template strand (3′ to 5′).

14
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Q: What does the genetic code being universal, redundant, and non-overlapping mean?

A: Same code for all life, multiple codons per amino acid, codons read one at a time.

15
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Q: What is the genome?

A: All of an organism’s genetic material.

16
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Q: How are prokaryotic chromosomes arranged?

A: Circular, in a nucleoid.

17
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Q: How are eukaryotic chromosomes arranged?

A: Linear, wrapped around histones → nucleosomes → chromatin fiber.

18
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Q: What is a nucleosome?

A: DNA wrapped around histones.

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Q: What do genes code for?

A: Polypeptides (proteins) or RNA.

20
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Q: What do exons and introns do?

A: Exons code for mature RNA; introns are removed.

21
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Q: How many chromosomes do humans have?

A: 46 total, 23 pairs.

22
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Q: What does diploid mean?

A: Two copies of each chromosome (2n).

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Q: What does haploid mean?

A: One copy of each chromosome (1n).

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Q: What is polyploidy?

A: More than two copies of each chromosome.

25
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Q: What is a homologous pair?

A: Pair from each parent with same genes but possibly different alleles.

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Q: What are autosomes?

A: Non-sex chromosomes.

27
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Q: What determines biological sex?

A: Sex chromosomes (XX = female, XY = male).

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Q: What is a sister chromatid?

A: Identical copy of a chromosome after DNA replication.

29
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Q: How many chromosomes does 1 pair of sister chromatids equal?

A: One chromosome until they separate.

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Q: When do sister chromatids become separate chromosomes?

A: After they are pulled apart in cell division.

31
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Q: Are homologous chromosomes and sister chromatids the same?

A: No!