Technique of the Week - Voltage Clamp

certain voltages

Using the voltage clamp technique allows us to discover what happens in the neuron at ______ ______

internal electrode

Step one: One ______ ______ measures membrane potential and is connected to the voltage clamp amplifier

desired potential

Step two: voltage clamp amplifier compares membrane potential with the _______ _______

the same

Step three: when Vm is different from the command potential, the clamp amplifier injects current into the axon through a second electrode, causing the membrane potential to become ___ ____ as the command potential

Hodgkin and Huxley

Which two neuroscientists used voltage clamp in a squid giant axon and discovered ionic basis of action potential

negative

By convention, positive charge entering the cell is plotted as ______ current