TCA cycle

TPP - Thiamine Pyrophosphate

Lipo- Lipoate

FAD- Flavin Adenine Dinucleotide

CoA- Coenzyme A

NAD- Nicotinamide Adenine Dinucleotide

Five coenzymes are required in the PD Enzyme complex

Acetyl-CoA + 3 NAD+ + FAD + GDP + P; + 2H2O >

CoA-SH + 3 NADH + FADH, + 3H. + GTP + 2CO2

Overall Reaction of TCA

Citrate Synthase

TCA Step 1 – Citrate Formation Enzyme is

NADH and Succinyl-CoA are allosteric inhibitors

Citrate Formation Inhibitors

Aconitase

TCA Step 2 –Isomerization to Isocitrate Enzyme

fluorocitrate

Aconitase Inhibitors

Oxidative Decarboxylation

TCA Step 3 – ___________________

Isocitrate dehydrogenase

TCA Step 3- Enzyme

Decarboxylation to Succinyl-CoA

TCA Step 4 – ___________________

TPP, CoASH,
lipoic acid, NAD+, and FAD

TCA Step 4:It requires 5 coenzymes:

a-ketoglutarate dehydrogenase

TCA Step 4 –enzyme

Conversion to Succinate

TCA Step 5 – __________________

GTP

Succinyl-CoA synthetase produces ____

Succinyl CoA synthase

TCA Step 5: enzyme

Oxidation of Succinate to Fumarate

TCA Step 6 – _________________________

FADH2

TCA Step 6:Oxidation of Succinate to Fumarate releases

Succinate Dehydrogenase

TCA Step 6:Oxidation of Succinate to Fumarate enzyme

Hydration of Fumarate to Malate

TCA Step 7 – _______________________

Fumarase

TCA Step 7 – Hydration of Fumarate to Malate enzyme

Oxidation of Malate to Oxaloacetate

TCA Step 8 – __________________________

energetically expensive: ΔGo' = +30 kJ/mol

TCA Step 8- This reaction is _____________________________

Malate Dehydrogenase

TCA step 8 Enzyme

ATP, NADH & Acetyl-CoA

NAD+ & CoA

Step 0: Pyruvate Dehydrogenase – Inhibited by

Activated by

ATP, NADH & Succinyl-CoA

Step 1: Citrate Synthase – Inhibited by

Inhibited by ATP

Activated by ADP and NAD+

Step 3: Isocitrate Dehydrogenase – Inhibited by

NADH & Succinyl-CoA

AMP

Step 4: a-Ketoglutarate Dehydrogenase – Inhibited by

Activated by