QA IN CLINICAL MICROSCOPY, BLOOD BANK, AND IMMUNOSERO

Clinical Microscopy

Branch of laboratory medicine that performs different methods to detect problems in the human body using body fluids other than blood

Refractometer

Instrument that measures specific gravity

Refractive index

Principle of refractometer; comparison of the velocity of light in air with the velocity of light in a solution

1.000

Calibration of distilled water in refractometer

1.021-1.023

Calibration of 5% NaCl in refractometer

1.033-1.035

Calibration of 9% sucrose in refractometer

Immunohematology

The study of antigens associated with the cellular components of the blood, the immunologic properties of blood components, and their implications in blood transfusion

Bombay blod

Rare blood type in India wherein H antigen is absent and H antibodies are present

Whole blood

Used to replace the loss of both RBC mass and plasma volume

1-6 C

Storage Temperature of Whole Blood

35 days

shelf life of whole blood with CPDA-1

21 days

Shelf life of whole blood without CPDA

72 hours

Whole blood must be stored for at least ____________________ to prevent syphylis

TRUE

TRUE OR FALSE: Whole blood must NOT be frozen unless there is glycerol present

Red blood cells

Also known as packed red cell

Centrifugation

RBCs are separated from plasma through _________________________

Anemic

RBCs are used commonly for _____________ patients

1-6C

Storage temperature of RBC

Random-donor platelet concentrate

Separation from plasma component of whole blood; must be prepared within 4 hours of collection

Single-donor platelet concentrate

Aprehesis

Thrombocytopenia

Platelet concentrate is often used for patients with ________________________________

20-24 C

Storage requirement for Platelet Concentrate with agitation

5.5 x 10^10, 50-70 mL

Count and volume of random donor platelet concentrate

3 x 10^11, 200-400 mL

Platelet count and volume of single donor platelet concentrate

5 days

shelf life of platelet concentrate

6-8 hours

Fresh frozen plasma must be prepared within ___________________ depending on anticoagulant used

-18 C

Storage requirement of FFP for 1 year

-65 C

Storage of FFP (7 years)

Cryoprecipitate

Prepared from centrifugation of FFP

Fibrinogen replacement

Purpose of cryoprecipitate

plasmapheresis

How is Factor VIII/IX Concentrate prepared?

Hemophilia

Factor VIII/IX Concentrate is used for patients with ______________________

Neutropenia

Granulocyte Concentrate is used for patients with severe _____________________

20-24 C

Storage requirement of granulocyte concentrate

24 hours

Shelf life of granulocyte concentrate

Serology

Study of serum

Serological test kits

Non-instrument based tests that are rapid and simple to perform; Need minute samples only (drops)

Lateral Flow Immunoassays

Also known as rapid diagnostic test kit

antigens; antibodies

As the sample moves along the membrane of the test kit, the conjugated ___________________ are captured by ________________

Control line

Excess dye conjugates, will continue to move along the

membrane, which will then be bound by antibodies at the

________________________ and form another visible line