Culturing Microorganisms / Preserving Bacteria

Inoculation

introducing microorganism into environment where they will grow and reproduce

Radial

place the inoculum at the center and bring to the other side in concentric manner

Overlap

streak the medium into three planes using cotton swab

Multiple

medium separated into several division then streak in each one

Interrupted

streak on one side then stop half way and streak again on the other side of the plate

Multiple interrupted

streak on the ¼ then stop

Pellicle

mass of microbes in the top of broth

sediment

mass of microbes at the bottom

Incubation

it produces culture — the viable growth of the microbe in or the medium

Incubator

can be used to create proper growth conditions for sample

Isolation

next step after incubating the samples

Sub-culturing term

it is used to describe the procedure of transferring of microbes by taking tiny bit of growth from isolated colony

Inspection

it involves steps including culturing, staining, and microscoping examination

Identification

fundamental aspect of microbiology that has broad implications for health, safety, and environment

-80 Celsius

High percentage of glycerol

bacteria can be preserved for months and years if stored at what temperature?

Colonial morphology

distinctive characteristics of colonies observed on the surface

Shape and size of colony

measure with millimeter rule less than 1mm is pin point

Edge or Margin of colony

edge shape of colony

Chromogenesis

ability of organism to produce color

Opacity of colony

if colony is transparent, opaque, or translucent

Elevation of colony

it can be convex, concave, doomed

Surface of colony

can be smooth, rough, dull etch

Consistency

colony may be buttery, viscid

Form

can be round, irregular, filamentous