Looks like no one added any tags here yet for you.
Favors the growth of organism of interest
Selective Media
Cystine Tellurite Blood Agar is selective for?
Corynebacterium diphtheriae
Inhibitor of Cystine Tellurite Blood Agar
Potassium Tellurite
Lowenstein Jensen Media is selective for?
Mycobacterium tuberculosis
Inhibitor of Lowenstein Jensen Media
Malachite green
Thayer Martin Media is selective for?
Neisseria gonorrhoeae
Inhibitor of THAYER MARTIN MEDIA
Vancomycin (gram +), Colistin (-), Nystatin (fungal inhibitor)
Inhibitor of MODIFIED THAYER MARTIN MEDIA
Vancomycin (+), Colistin (-), Nystatin (fungal inhibitor), Trimethoprim lactate (proteus)
Inhibitor of Martin Lewis Media
Vancomycin (+), Colistin (-), Anicomycin (fungal inhibitor), Trimethoprim lactate (proteus)
Inhibitor for New York City Media
Vancomycin (+), Colistin (-), Amphotericin B (fungal inhibitors), Trimethoprim lactate (proteus)
Inhibitor for GC-LECT
Small of amount of Vancomycin (+), Lympovycin (++), Colistin (-), Amphotericin B (fungal inhibitors), Trimethoprim lactate (proteus)
Bismuth Sulfite Agar is selective for?
Salmonella species
Inhibitor of Bismuth Sulfite Agar
Bismuth Sulfite
In bismuth sulfite agar, _______ has a characteristic colonies which is black colonies with silver metallic sheen
Salmonella typhi
Brilliant Green Agar is selective for?
Salmonella species
This agar is selective for Salmonella species EXCEPT FOR SALMONELLA TYPHI
Brilliant Green Agar
Which bacteria has a charactertistic of white colonies resembling snowflakes surrounded by a brilliant red agar
Salmonella species
TRUE OR FALSE. Differential media contains inhibitors used to differentiate bacteria growing together
FALSE
Original color of Eosin Methylene Blue AGAR
Dark violet
Selective for of Eosin Methylene Blue AGAR
Gram Negative Enteric Bacilli
Inhibitors of Eosin Methylene Blue AGAR
Eosin and Methylene Blue
What are the 3 bacteria that has a characteristic growth in EMB?
E. coli, Enterobacter, Klebsiella
What bacteria has a characteristic of dark pink/violet with green metallic sheen
E. coli
What bacteria has a characteristic of dark pink/violet wherein there may or may not have black center
Enterobacter
What bacteria has a characteristic of dark pink/violet, large, mucoid
Klebsiella
Original color of Mac Conkey Agar
Salmon pink
Selective of Mac Conkey Agar
Gram negative enteric bacilli
Inhibitors of Mac Conkey Agar
Crystal violet, bile salts, citrate
Indicators of Mac Conkey Agar
Neutral red
Lactose fermenter in Mac Conkey Agar produces what color?
Pink
NON Lactose fermenter in Mac Conkey Agar produces what color?
Colorless
What agar has an original color of light orange?
Salmonella Shigella Agar (SSA)
Inhibitors of Salmonella-Shigella AGAR
Brilliant green, Bile salts, citrate
pH indicator of Salmonella-shigella agar
Neutral red
Presence of hydrogen sulfide may produce what?
Blackening
H2S indicator
Ferric Ammonium Citrate
Sulfur source?
Sodium Thiosulfate
Lactose fermenters in Salmonella-shigella agar
Dark pink
Both salmonella and shigella are non-lactose fermenters EXCEPT FOR WHAT?
Salmonella arizonae and Shigella sonnei
Among Shigella and Salmonella, which of them produces H2S?
Salmonella
Original color of Hektoen Enteric Agar
Dark Green
pH indicator of Hektoen Enteric Agar
Bromthymol blue
Lactose fermenter in HEA produces what color
Orange
Original color of Thiosulfate Citrate Bile Salts Sucrose Agar
Light green or olive green
TCBS agar is selective for?
Vibrio species
Sucrose fermenter are seen in what agar?
Thiosulfate Citrate Bile Salts Sucrose Agar
Sucrose fermenters produces what color?
Yellow
Non-sucrose fermenters produced what color?
Blue-green
What is used in testing Haemophilus species in media for AST
Haemophilus Test Media
Alternative for detecting Haemophilus spp. in media for AST
MHA + CAP as base
Media for AST used in detecting Mycobacteria species
Middlebrook 7H10 and 7H11
AST media for Methicillin Resistant S. aureus
MHA + 7.5% NaCl
AST media for Streptococcus species
MHA + 5% Sheep’s blood
AST method utilizing Mueller Hinton Agar is called
Kirby Bauer Method
Transport media for S. agalactiae
Todd Hewitt
Transport media for Neisseria species
Transgrow
General transport media
Amies
Amies is mostly used for what sample?
Respiratory
Bacterial transport media as well as viral transport media
Stuarts
What transport media is used for fastidious organisms/bacteria
Stuarts
Transport media for stool specimen (rectal swab)
Cary blair
Cary blair is used for transport media for ____ which is the causative agent of Cholera
Vibrio species
Transport media for N. gonorrhoeae
JEMBEC
Transport media is a type of media that is used to maintain the _____ of the microorganism during transport
Viability
This contains basic nutritional requirement to support the growth of non-fastidious organisms with additives, enriching substances, or supplements to support the growth of fastidious microorganisms
Enriched Culture Media
Blood agar plate and chocolate agar plate is an example of?
Enriched Culture Media
Which Culture Media has an X factor and V factor
Chocolate Agar Plate
Which Culture Media has an X factor
Blood Agar Plate
This is an enrichment culture media for S. agalactiae
Lim broth
The specimen used for S. agalactiae for lim broth is?
Vaginal Swab Specimen
Pregnant women are recommended to be screened for S. agalactiae because it is a common cause of?
Neonatal meningitis
What is the content of lim broth?
Todd Hewitt Broth and Colistin and Nalidixic acid
Todd Hewitt in Enrichment Culture Media is used for?
Beta-hemolytic streptococcus, Streptococcus pneumoniae
Gram Negative broth is used for?
Salmonella and Shigella
What enrichment culture media is used for Anaerobic bacteria
Thioglycolate Broth
What enrichment culture media is used for Salmonella species?
Selenite F broth
What enrichment culture media is used for Vibrio species?
Alkaline Peptone Water
This enhances the growth of a particular bacteria
Enrichment Culture Media
This formation is solidified in a petri dish
Plated
Standard size of Petri Dish
100 mm
How much culture media is used in a 100 mm petri dish
15-20 mL
When is 150 mm petri dish used?
Antibiotic Susceptibility Test
Manner of inoculation of Broth
Mixing
Manner of inoculation of Butt Semi-solid
Stab
Manner of inoculation of slant
Streak
Manner of inoculation of butt-slant
Butt:Stab, Slant:Streak
Bottled formation are most likely to be used for?
Blood Culture
Type of physical state wherein there are 2 physical state in one culture media
Biphasic
Rice media, potato slices, and cooked meat are an example of?
Solid non-liquefiable
How much agar is added to form a solid culture media?
2-3%
The most used physical state in preparing culture media is?
SOLID
When performing motility test, a bacteria that is steady and till from the site of inoculation means what?
Negative in motility
When performing motility test, spreading of bacteria from the site of inoculation means?
Presence of motility
What is the media used in demonstrating motility?
Sulfide Indole Motility Media
How much agar is added to form a semi-solid culture media?
0.5-1.0%
Semi solid culture media are mostly useful in studying?
motility
Detection for the presence of growth in broth culture media is by checking the?
Turbidity
Broth culture media are commonly used for?
Rapid culture
Solidifying agent used in culture media is?
Agar/agarose
These are composed of kidney cells of African Green Monkey
Vero Cell Line