CULTURE MEDIA EME

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Favors the growth of organism of interest

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1

Favors the growth of organism of interest

Selective Media

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2

Cystine Tellurite Blood Agar is selective for?

Corynebacterium diphtheriae

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3

Inhibitor of Cystine Tellurite Blood Agar

Potassium Tellurite

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4

Lowenstein Jensen Media is selective for?

Mycobacterium tuberculosis

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5

Inhibitor of Lowenstein Jensen Media

Malachite green

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6

Thayer Martin Media is selective for?

Neisseria gonorrhoeae

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7

Inhibitor of THAYER MARTIN MEDIA

Vancomycin (gram +), Colistin (-), Nystatin (fungal inhibitor)

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8

Inhibitor of MODIFIED THAYER MARTIN MEDIA

Vancomycin (+), Colistin (-), Nystatin (fungal inhibitor), Trimethoprim lactate (proteus)

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9

Inhibitor of Martin Lewis Media

Vancomycin (+), Colistin (-), Anicomycin (fungal inhibitor), Trimethoprim lactate (proteus)

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10

Inhibitor for New York City Media

Vancomycin (+), Colistin (-), Amphotericin B (fungal inhibitors), Trimethoprim lactate (proteus)

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11

Inhibitor for GC-LECT

Small of amount of Vancomycin (+), Lympovycin (++), Colistin (-), Amphotericin B (fungal inhibitors), Trimethoprim lactate (proteus)

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12

Bismuth Sulfite Agar is selective for?

Salmonella species

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13

Inhibitor of Bismuth Sulfite Agar

Bismuth Sulfite

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14

In bismuth sulfite agar, _______ has a characteristic colonies which is black colonies with silver metallic sheen

Salmonella typhi

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15

Brilliant Green Agar is selective for?

Salmonella species

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16

This agar is selective for Salmonella species EXCEPT FOR SALMONELLA TYPHI

Brilliant Green Agar

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17

Which bacteria has a charactertistic of white colonies resembling snowflakes surrounded by a brilliant red agar

Salmonella species

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18

TRUE OR FALSE. Differential media contains inhibitors used to differentiate bacteria growing together

FALSE

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19

Original color of Eosin Methylene Blue AGAR

Dark violet

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20

Selective for of Eosin Methylene Blue AGAR

Gram Negative Enteric Bacilli

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21

Inhibitors of Eosin Methylene Blue AGAR

Eosin and Methylene Blue

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22

What are the 3 bacteria that has a characteristic growth in EMB?

E. coli, Enterobacter, Klebsiella

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23

What bacteria has a characteristic of dark pink/violet with green metallic sheen

E. coli

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24

What bacteria has a characteristic of dark pink/violet wherein there may or may not have black center

Enterobacter

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25

What bacteria has a characteristic of dark pink/violet, large, mucoid

Klebsiella

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26

Original color of Mac Conkey Agar

Salmon pink

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27

Selective of Mac Conkey Agar

Gram negative enteric bacilli

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28

Inhibitors of Mac Conkey Agar

Crystal violet, bile salts, citrate

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29

Indicators of Mac Conkey Agar

Neutral red

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30

Lactose fermenter in Mac Conkey Agar produces what color?

Pink

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31

NON Lactose fermenter in Mac Conkey Agar produces what color?

Colorless

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32

What agar has an original color of light orange?

Salmonella Shigella Agar (SSA)

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33

Inhibitors of Salmonella-Shigella AGAR

Brilliant green, Bile salts, citrate

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34

pH indicator of Salmonella-shigella agar

Neutral red

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35

Presence of hydrogen sulfide may produce what?

Blackening

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36

H2S indicator

Ferric Ammonium Citrate

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37

Sulfur source?

Sodium Thiosulfate

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38

Lactose fermenters in Salmonella-shigella agar

Dark pink

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39

Both salmonella and shigella are non-lactose fermenters EXCEPT FOR WHAT?

Salmonella arizonae and Shigella sonnei

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40

Among Shigella and Salmonella, which of them produces H2S?

Salmonella

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41

Original color of Hektoen Enteric Agar

Dark Green

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42

pH indicator of Hektoen Enteric Agar

Bromthymol blue

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43

Lactose fermenter in HEA produces what color

Orange

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44

Original color of Thiosulfate Citrate Bile Salts Sucrose Agar

Light green or olive green

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45

TCBS agar is selective for?

Vibrio species

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46

Sucrose fermenter are seen in what agar?

Thiosulfate Citrate Bile Salts Sucrose Agar

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47

Sucrose fermenters produces what color?

Yellow

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48

Non-sucrose fermenters produced what color?

Blue-green

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49

What is used in testing Haemophilus species in media for AST

Haemophilus Test Media

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50

Alternative for detecting Haemophilus spp. in media for AST

MHA + CAP as base

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51

Media for AST used in detecting Mycobacteria species

Middlebrook 7H10 and 7H11

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52

AST media for Methicillin Resistant S. aureus

MHA + 7.5% NaCl

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53

AST media for Streptococcus species

MHA + 5% Sheep’s blood

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54

AST method utilizing Mueller Hinton Agar is called

Kirby Bauer Method

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55

Transport media for S. agalactiae

Todd Hewitt

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56

Transport media for Neisseria species

Transgrow

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57

General transport media

Amies

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58

Amies is mostly used for what sample?

Respiratory

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59

Bacterial transport media as well as viral transport media

Stuarts

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60

What transport media is used for fastidious organisms/bacteria

Stuarts

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61

Transport media for stool specimen (rectal swab)

Cary blair

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62

Cary blair is used for transport media for ____ which is the causative agent of Cholera

Vibrio species

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63

Transport media for N. gonorrhoeae

JEMBEC

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64

Transport media is a type of media that is used to maintain the _____ of the microorganism during transport

Viability

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65

This contains basic nutritional requirement to support the growth of non-fastidious organisms with additives, enriching substances, or supplements to support the growth of fastidious microorganisms

Enriched Culture Media

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66

Blood agar plate and chocolate agar plate is an example of?

Enriched Culture Media

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67

Which Culture Media has an X factor and V factor

Chocolate Agar Plate

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68

Which Culture Media has an X factor

Blood Agar Plate

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69

This is an enrichment culture media for S. agalactiae

Lim broth

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70

The specimen used for S. agalactiae for lim broth is?

Vaginal Swab Specimen

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71

Pregnant women are recommended to be screened for S. agalactiae because it is a common cause of?

Neonatal meningitis

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72

What is the content of lim broth?

Todd Hewitt Broth and Colistin and Nalidixic acid

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73

Todd Hewitt in Enrichment Culture Media is used for?

Beta-hemolytic streptococcus, Streptococcus pneumoniae

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74

Gram Negative broth is used for?

Salmonella and Shigella

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75

What enrichment culture media is used for Anaerobic bacteria

Thioglycolate Broth

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76

What enrichment culture media is used for Salmonella species?

Selenite F broth

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77

What enrichment culture media is used for Vibrio species?

Alkaline Peptone Water

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78

This enhances the growth of a particular bacteria

Enrichment Culture Media

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79

This formation is solidified in a petri dish

Plated

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80

Standard size of Petri Dish

100 mm

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81

How much culture media is used in a 100 mm petri dish

15-20 mL

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82

When is 150 mm petri dish used?

Antibiotic Susceptibility Test

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83

Manner of inoculation of Broth

Mixing

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84

Manner of inoculation of Butt Semi-solid

Stab

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85

Manner of inoculation of slant

Streak

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86

Manner of inoculation of butt-slant

Butt:Stab, Slant:Streak

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87

Bottled formation are most likely to be used for?

Blood Culture

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88

Type of physical state wherein there are 2 physical state in one culture media

Biphasic

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89

Rice media, potato slices, and cooked meat are an example of?

Solid non-liquefiable

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90

How much agar is added to form a solid culture media?

2-3%

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91

The most used physical state in preparing culture media is?

SOLID

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92

When performing motility test, a bacteria that is steady and till from the site of inoculation means what?

Negative in motility

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93

When performing motility test, spreading of bacteria from the site of inoculation means?

Presence of motility

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94

What is the media used in demonstrating motility?

Sulfide Indole Motility Media

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95

How much agar is added to form a semi-solid culture media?

0.5-1.0%

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96

Semi solid culture media are mostly useful in studying?

motility

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97

Detection for the presence of growth in broth culture media is by checking the?

Turbidity

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98

Broth culture media are commonly used for?

Rapid culture

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99

Solidifying agent used in culture media is?

Agar/agarose

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100

These are composed of kidney cells of African Green Monkey

Vero Cell Line

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