CULTURE MEDIA EME

Favors the growth of organism of interest

Selective Media

Cystine Tellurite Blood Agar is selective for?

Corynebacterium diphtheriae

Inhibitor of Cystine Tellurite Blood Agar

Potassium Tellurite

Lowenstein Jensen Media is selective for?

Mycobacterium tuberculosis

Inhibitor of Lowenstein Jensen Media

Malachite green

Thayer Martin Media is selective for?

Neisseria gonorrhoeae

Inhibitor of THAYER MARTIN MEDIA

Vancomycin (gram +), Colistin (-), Nystatin (fungal inhibitor)

Inhibitor of MODIFIED THAYER MARTIN MEDIA

Vancomycin (+), Colistin (-), Nystatin (fungal inhibitor), Trimethoprim lactate (proteus)

Inhibitor of Martin Lewis Media

Vancomycin (+), Colistin (-), Anicomycin (fungal inhibitor), Trimethoprim lactate (proteus)

Inhibitor for New York City Media

Vancomycin (+), Colistin (-), Amphotericin B (fungal inhibitors), Trimethoprim lactate (proteus)

Inhibitor for GC-LECT

Small of amount of Vancomycin (+), Lympovycin (++), Colistin (-), Amphotericin B (fungal inhibitors), Trimethoprim lactate (proteus)

Bismuth Sulfite Agar is selective for?

Salmonella species

Inhibitor of Bismuth Sulfite Agar

Bismuth Sulfite

In bismuth sulfite agar, _______ has a characteristic colonies which is black colonies with silver metallic sheen

Salmonella typhi

Brilliant Green Agar is selective for?

Salmonella species

This agar is selective for Salmonella species EXCEPT FOR SALMONELLA TYPHI

Brilliant Green Agar

Which bacteria has a charactertistic of white colonies resembling snowflakes surrounded by a brilliant red agar

Salmonella species

TRUE OR FALSE. Differential media contains inhibitors used to differentiate bacteria growing together

FALSE

Original color of Eosin Methylene Blue AGAR

Dark violet

Selective for of Eosin Methylene Blue AGAR

Gram Negative Enteric Bacilli

Inhibitors of Eosin Methylene Blue AGAR

Eosin and Methylene Blue

What are the 3 bacteria that has a characteristic growth in EMB?

E. coli, Enterobacter, Klebsiella

What bacteria has a characteristic of dark pink/violet with green metallic sheen

E. coli

What bacteria has a characteristic of dark pink/violet wherein there may or may not have black center

Enterobacter

What bacteria has a characteristic of dark pink/violet, large, mucoid

Klebsiella

Original color of Mac Conkey Agar

Salmon pink

Selective of Mac Conkey Agar

Gram negative enteric bacilli

Inhibitors of Mac Conkey Agar

Crystal violet, bile salts, citrate

Indicators of Mac Conkey Agar

Neutral red

Lactose fermenter in Mac Conkey Agar produces what color?

Pink

NON Lactose fermenter in Mac Conkey Agar produces what color?

Colorless

What agar has an original color of light orange?

Salmonella Shigella Agar (SSA)

Inhibitors of Salmonella-Shigella AGAR

Brilliant green, Bile salts, citrate

pH indicator of Salmonella-shigella agar

Neutral red

Presence of hydrogen sulfide may produce what?

Blackening

H2S indicator

Ferric Ammonium Citrate

Sulfur source?

Sodium Thiosulfate

Lactose fermenters in Salmonella-shigella agar

Dark pink

Both salmonella and shigella are non-lactose fermenters EXCEPT FOR WHAT?

Salmonella arizonae and Shigella sonnei

Among Shigella and Salmonella, which of them produces H2S?

Salmonella

Original color of Hektoen Enteric Agar

Dark Green

pH indicator of Hektoen Enteric Agar

Bromthymol blue

Lactose fermenter in HEA produces what color

Orange

Original color of Thiosulfate Citrate Bile Salts Sucrose Agar

Light green or olive green

TCBS agar is selective for?

Vibrio species

Sucrose fermenter are seen in what agar?

Thiosulfate Citrate Bile Salts Sucrose Agar

Sucrose fermenters produces what color?

Yellow

Non-sucrose fermenters produced what color?

Blue-green

What is used in testing Haemophilus species in media for AST

Haemophilus Test Media

Alternative for detecting Haemophilus spp. in media for AST

MHA + CAP as base

Media for AST used in detecting Mycobacteria species

Middlebrook 7H10 and 7H11

AST media for Methicillin Resistant S. aureus

MHA + 7.5% NaCl

AST media for Streptococcus species

MHA + 5% Sheep’s blood

AST method utilizing Mueller Hinton Agar is called

Kirby Bauer Method

Transport media for S. agalactiae

Todd Hewitt

Transport media for Neisseria species

Transgrow

General transport media

Amies

Amies is mostly used for what sample?

Respiratory

Bacterial transport media as well as viral transport media

Stuarts

What transport media is used for fastidious organisms/bacteria

Stuarts

Transport media for stool specimen (rectal swab)

Cary blair

Cary blair is used for transport media for ____ which is the causative agent of Cholera

Vibrio species

Transport media for N. gonorrhoeae

JEMBEC

Transport media is a type of media that is used to maintain the _____ of the microorganism during transport

Viability

This contains basic nutritional requirement to support the growth of non-fastidious organisms with additives, enriching substances, or supplements to support the growth of fastidious microorganisms

Enriched Culture Media

Blood agar plate and chocolate agar plate is an example of?

Enriched Culture Media

Which Culture Media has an X factor and V factor

Chocolate Agar Plate

Which Culture Media has an X factor

Blood Agar Plate

This is an enrichment culture media for S. agalactiae

Lim broth

The specimen used for S. agalactiae for lim broth is?

Vaginal Swab Specimen

Pregnant women are recommended to be screened for S. agalactiae because it is a common cause of?

Neonatal meningitis

What is the content of lim broth?

Todd Hewitt Broth and Colistin and Nalidixic acid

Todd Hewitt in Enrichment Culture Media is used for?

Beta-hemolytic streptococcus, Streptococcus pneumoniae

Gram Negative broth is used for?

Salmonella and Shigella

What enrichment culture media is used for Anaerobic bacteria

Thioglycolate Broth

What enrichment culture media is used for Salmonella species?

Selenite F broth

What enrichment culture media is used for Vibrio species?

Alkaline Peptone Water

This enhances the growth of a particular bacteria

Enrichment Culture Media

This formation is solidified in a petri dish

Plated

Standard size of Petri Dish

100 mm

How much culture media is used in a 100 mm petri dish

15-20 mL

When is 150 mm petri dish used?

Antibiotic Susceptibility Test

Manner of inoculation of Broth

Mixing

Manner of inoculation of Butt Semi-solid

Stab

Manner of inoculation of slant

Streak

Manner of inoculation of butt-slant

Butt:Stab, Slant:Streak

Bottled formation are most likely to be used for?

Blood Culture

Type of physical state wherein there are 2 physical state in one culture media

Biphasic

Rice media, potato slices, and cooked meat are an example of?

Solid non-liquefiable

How much agar is added to form a solid culture media?

2-3%

The most used physical state in preparing culture media is?

SOLID

When performing motility test, a bacteria that is steady and till from the site of inoculation means what?

Negative in motility

When performing motility test, spreading of bacteria from the site of inoculation means?

Presence of motility

What is the media used in demonstrating motility?

Sulfide Indole Motility Media

How much agar is added to form a semi-solid culture media?

0.5-1.0%

Semi solid culture media are mostly useful in studying?

motility

Detection for the presence of growth in broth culture media is by checking the?

Turbidity

Broth culture media are commonly used for?

Rapid culture

Solidifying agent used in culture media is?

Agar/agarose

These are composed of kidney cells of African Green Monkey

Vero Cell Line