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Open reading frames are
capable of encoding proteins based on sequence
ORFs are
difficult to identify in eukaryotic genomes
Coding regions are
broken up into separate exons
Each exon does not have
its own start codon and stop codon
Each exon is
not in the same “frame” as the others
• introns are not confined to lengths of multiples-of-3
Exons are, compared to introns
very short compared to introns
Gene sequences will have
many possible ORFs that are in introns
Processed mRNAs have
three possible reading frames