Microbio Chapter 1

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How are prokaryotes able to live in such harsh environments?

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1

How are prokaryotes able to live in such harsh environments?

They are very adaptable and resistant

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2

Mutualism

A relationship between two species in which both species benefit

Gut nutrient metabolism

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3

Amensalism

a relationship in which one organism is harmed and the other is unaffected

Antimicrobial defense on skin

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Commensalism

A relationship between two organisms in which one organism benefits and the other is unaffected

Skin cells as food source

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Neutralism

Neither species benefits or is harmed

Spores in soil

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Parasitism

A relationship between two organisms of different species where one benefits and the other is harmed

Tuberculosis and leprosy

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How do prokaryotes reproduce?

binary fission

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What are the examples of gram-negative prokaryotes?

Proteobacteria Spirochete CFB group Planctomycetes] Phototrophic bacteria

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What are the types of gram-positive prokaryotes?

Actinobacteria Firmicutes

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10

What is the atypical kind of bacteria?

Tenericutes

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11

What are kinds of proteobacteria?

Alphaproteobacteria Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria

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12

What are all Alphaproteobacteria?

Oligotrophs

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Oligotrophs

bacteria capable of living in low nutrient environments

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14

Prominent genera of alphaproteobacteria

Rickettsia spp. Chlamydia spp.

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15

What does Rickettsia cause?

causative agents for Rocky mountain spotted fever & typhus fevers​

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What does Chlamydia cause?

causative agents ​for lymphogranuloma ​venereum (STD)​

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What do Rickettsia and Chlamydia have in common?

they are both obligate intracellular and must have host to be metabolically active

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18

What are all betaproteobacteria?

Eutrophs

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19

Eutrophs

require a copious amount of organic nutrients

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Prominent genera of betaproteobacteria

Bordetella spp. Neisseria gonorrhea Neisseria meningitides

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What does Bordetella cause?

causative agents whooping cough (pertussis) & kennel cough;​produces toxins to paralyze lung cilia

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What does Neisseria gonorrhea cause?

causative agent for gonorrhea STD​

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What does Neisseria meningitides cause?

causative agent for bacterial meningitis​

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24

Prominent genera of gammaproteobacteria

Pseudomonas Pasteurella Haemophilus Vibrio Legionella Enterobacter family

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What does Pseudomonas aeruginosa cause?

common infection of wounds, urinary tract, & respiratory tract

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What does Pasteurella haemolytica cause?

causative agent for severe pneumonia in animals​

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What does Haemophilus influenzae cause?

causative agent for upper & lower respiratory infections (does not cause influenza)​

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What is special about the Vibrio spp.?

commonly found in alkaline environments such as ocean ports & lagoons

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What does Vibrio cholerae cause?

causative agent of cholera and common to water contamination​

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What does Legionella pneumophila cause?

causative agent for Legionnaire's disease and common to water contamination​

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31

What are groups of the enterobacter family?

coliforms and noncoliforms

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Coliforms

ferment lactose with acid and gas production

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Noncoliforms

fermentation of lactose is incomplete or absent

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What are some bacteria from the enterobacter family?

includes E. coli, & Salmonella spp.

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What is special about deltaproteobatceria?

Sulfate reducing bacteria​

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What are prominent genera of deltaproteobacteria?

Desulfovibrio orale Parasitic Bdellovibrio spp. Myxobacteria

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What does Desulfovibrio orale cause?

periodontal disease

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What is Myxobacteria?

soil dwelling "slime bacteria"

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39

What are prominent genera of epsilonproteobacteria?

Campylobacter Helicobacter

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40

What does the Campylobacter spp. cause?

common to food poisoning

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What does the Helicobacter spp. cause?

commonly beneficial but can cause ulcers and stomach cancer in susceptible people

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42

What are the gram-negative nonproteobacteria?

Spirochetes The CFB group Planctomycetes

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What is special about spirochetes?

Gram (-) extremely thin and hard to stain & culture have axial filament similar to flagella

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What are some types of spirochetes?

Treponema pallidum Borrelia burgdorferi

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What does Treponema pallidum cause?

causative agent for​syphilis

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What does Borrelia burgdorferi cause?

Lyme disease

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Who makes up the CFB group?

Cytophaga spp. Fusobacteria spp. Bacteroides spp.

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What does Cytophaga spp. have?

gliding system for motility (mechanisms mostly unknown)​

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What does Fusobacteria spp. cause?

inhabit mouth and can cause various oral diseases

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What is significant about Bacteroides spp.?

30% of gut microbiome; lower levels correlated with obesity

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What is significant about planctomycetes?

Found in aquatic environments: fresh, salt, and brackish​ Reproduce via budding instead of binary fission​ Sessile cells - immobile with holdfast appendage (a)​ Swarmer cells - motile, unable to reproduce (b)

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What is significant about phototrophic bacteria

Utilize sunlight as main source of energy via photosynthesis​ Oxygenic - produce O2​ Anoxygenic - do not produce O2​

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53

What is an example of an oxygenic photosynthesizer?

Cyanobacteria

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54

What is significant about cyanobacteria?

highly adaptable and diverse; chlorophyll; uses as biosorbents and human nutrition

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What is an example of a cyanobacteria?

Microsystis spp.

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What do Microsystis spp. cause?

toxic algal blooms

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What are the types of anoxygenic photosynthesizers?

Purple Purple non-sulfur Green Green non-sulfur

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58

What are types of gram-positive bacteria?

Actinobacteria Firmicutes

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How do you put a gram-positive bacteria into either Actinobacteria or Firmicutes?

Grouping by guanine + cytosine content​ Phylum Actinobacteria - high G+C content (>50%)​ Phylum Firmicutes - low G+C content (<50%)

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What are Actinobacteria?

High G+C gram positive bacteria Extremely diverse​ Thin, filamentous to coccobacilli shaped​ Important to soil ecology​ Different peptidoglycans in cell wall​

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What are the prominent genera for actinobacteria?

Mycobacterium ​ Corynebacterium ​ Bifidobacterium​ Gardnerella

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What does Mycobacterium spp. cause?

causative agent for tuberculosis & leprosy Acid fast (+) due to mycolic acid in cell wall

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What does Corynebacterium spp. cause?

Most are non-pathogenic; ​C. diphtheria is causative agent for diphtheria Diaminopimelic acids in cell wall

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What is special about Bifidobacterium?

Filamentous & anaerobic​ Frequently used as probiotic Extremely good for your gut

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What does Gardnerella vaginalis cause?

Causative agent for bacterial vaginosis Gram variable: inconsistent stain results

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What is common among all firmicutes?

low G+C content​

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What are the prominent genera of firmicutes?

Clostridium​ Streptococcus​ Lactobacillus​ Enterococcus​ Bacillus​ Staphylococcus

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What is special about clostridium?

Endospore producers​ Soil dwelling​ Common food contaminant

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What does C. perfringens cause?

agent for food poisoning and gangrene

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What does C. tetani cause?

producer of neurotoxin and agent for tetanus​

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What does C. botulinum cause?

producer of botulinum neurotoxin

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What does C. difficile cause?

hospital infection, causes severe colitis​

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What are some kinds of lactobacillales?

Streptococcus​ Lactobacillus​ Enterococcus

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74

Lactobacillales contain?

bacilli and cocci

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What does Streptococcus pyogenes cause?

β-hemolytic cocci associated with pus production (pyogenic), strep throat

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What does Streptococcus pneumoniae cause?

causes pneumonia, respiratory infections, and a wide range of other diseases

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What is special about lactobacillus?

Facultative anaerobes​ non-spore formers​ significant component of gut ​microbiome​ starter cultures for ​yogurt, cheese, sauerkraut, etc.

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What is special about enterococcus?

Diplicocci arrangement​ Anaerobic respiration​ Commensal gut microbe​ Common UTI pathogen​ E. faecium

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What is special about Bacillus?

bacillus shaped aerobes or facultative anaerobes​ Endospore producers​ Important to industrial microbiology

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What does B. anthracis cause?

causative agent for anthrax

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What does B. cereus cause?

common food poisoning agent​

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What does B. thuringiensis cause?

producer of insecticide compounds​

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What is special about staphylococcus?

cocci shaped facultative anaerobes​ Halophilic​ Nonmotile

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What does S. aureus cause?

common agent of skin infections; some can produce enterotoxins for food poisoning​Some are very antibiotic resistant (MRSA & VRSA)​

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85

What does S. epidermidis cause?

common flora on skin; can cause infection to open wounds​

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86

What is special about mycoplasma?

no cell wall and do not retain crystal violet​ (tenericute) pleomorphic​ Extremely small​ Cell wall antibiotics do not work​ Only classified by genome​

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What does M. pneumoniae cause?

agent for walking pneumonia​

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What are deeply branching bacteria?

Genera and species that are most common to the last universal common ancestor (LUCA)

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What are the prominent classes of the deeply branching bacteria?

Aquificae Thermotogae Deinococci

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What are Aquificae?

gram (-) hyperthermophiles living in hot springs and oven vents

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What are Thermotogae?

gram (-), hyperthermophilic anaerobes; sheath-like outer membrane

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What are Deinococci?

gram (+) polyextremophile (heat, vacuum, acidity tolerant)

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What are archaea?

Membranes with branched isoprene chains & ether linkages to phosphate head​ Walls of surface layer proteins​(some pseudopeptidoglycan)​ Genomes are much larger ​ Capable of methanogenesis​ Usually not in human microbiota​ Not directly associated with infectious diseases​

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What is the archaea membrane made of?

Esther linkages branched tails

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95

What are some phylum of archaea?

Crenarchaeota​ Euryarchaeota

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96

What is special about crenarchaeota?

All aquatic microbes, many extremophiles

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What is special about Sulfolobus spp.?

thermophiles & acidophiles; facultative anaerobic; used in biotech for production of affitins

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What is special about Thermoproteus spp.?

strict anaerobic thermophiles; arguably deepest branching Archaea

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What is special about Euryarchaeota?

mostly methanogens & anaerobes

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What are some methanogens?

Methanobacteria​ Methanococci​ Methanomicrobia

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