The Microscope Diagram | Quizlet

Ocular lens magnification?

10x

Objective lens magnifications?

4x, 10x, 40x, 100x

Formula to calculate total magnification?

ocular lens x objective lens

Condenser function?

Controls the amount of light that comes from the light source

Diaphragm function (microscope)?

Controls the amount of light that passes through slide

How do we transport the microscope?

2 hands

*hold arm and the base

How do we clean the lenses? (other than oil immersion)

only use lens paper

*no paper towels

When do we use oil immersion? What need to be done cleaning wise?

Only on the 100x objective

=always clean off the lens and don't go backwards

-it can damage other lenses

What should we do the voltage before turning on the microscope?

Make sure it is on low or off before turning on the microscope

What is resolution?

ability to completely separate two objects in the microscopic field

What is parfocal?

ability to change from low power to high power objectives with only fine focus adjustment

*do this at 100x and 40x

How does wavelength affect resolution?

shorter wavelength = greater resolution

what color filters does a microscope use? why?

Blue filter

*has the lowest wavelength of all visible light

-creates the best resolution

What are the nicknames for the objective lenses

10x

*low power

40x

*high dry

100x

*oil immersion lens

Why does oil help increase resolution?

Since oil has same refractory index as glass, it eliminates the refraction index

*allows for very high magnification (1000x) and still good resolution

What are the 5 broad steps of finding things in the microscope?

1. clean off the lenses

2. find a reference point

3. start on low power (10x)

4. switch to high power (40x)

5. switch to oil power (100x)

How can one find a reference point?

looks for clumps or the edge of the cover slip

*can also draw a circle on the slide

What should be done with finding things on low power?

Start with the stage all the way done and slowly move up

*move the stage to make sure sample is in focus (and not just part of the microscope)

What should be done at high dry power?

Only use the fine adjustment knob

*change the condenser to the objective (40x)

What can be done on 100x power?

Add oil and only use the fine adjustment

*change the condenser

Voltage control function

Controls amount of light coming out of the lamp

What controls the stage horizontal movement?

Mechanical stage adjustment knobs