MEMORIZE CULTURE MEDIAS
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Last updated 3:54 AM on 8/25/25
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146 Terms
1
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What is the classification of MacConkey Agar?
Selective and Differential
2
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What is the pH indicator in MacConkey Agar?
Neutral Red
3
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What inhibits Gram-positive bacteria in MacConkey Agar?
Crystal Violet
4
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What is the base composition of MacConkey Agar?
Peptone with Lactose
5
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What does a pink colony on MacConkey Agar indicate?
Lactose fermenter
6
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What does a colorless or white colony on MacConkey Agar indicate?
Non lactose fermenter
7
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What is the use of MacConkey Agar?
Isolation and differentiation of Gram-negative lactose and nonlactose fermenters
8
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What is the classification of Eosin Methylene Blue (EMB) Agar?
Selective and Differential
9
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What are the indicators in EMB Agar?
Eosin Y and Methylene Blue
10
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What inhibits Gram-positive bacteria in EMB Agar?
Eosin Y and Methylene Blue
11
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What sugars are present in EMB Agar?
Lactose and Sucrose
12
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What does a blue black colonies with a metallic green sheen colony on EMB Agar indicate?
Lactose Fermenter
13
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What does a pink colony on EMB Agar indicate?
Slow fermenter
14
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What does a colorless or amber colony on EMB Agar indicate?
Non fermenters
15
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What is the use of EMB Agar?
Isolation and differentiation of Gram-negative lactose and nonlactose fermenters
16
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What is the classification of Blood Agar?
Enriched and Differential
17
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What is the inhibitor in Blood Agar?
None
18
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What are the components of Blood Agar?
Tryptone, sodium chloride, 5% sheep or horse blood
19
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What does beta hemolysis on Blood Agar indicate?
Complete hemolysis
20
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What does alpha hemolysis on Blood Agar indicate?
Partial hemolysis
21
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What does gamma hemolysis on Blood Agar indicate?
No hemolysis
22
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What is the use of Blood Agar?
Cultivation of nonfastidious bacteria and determination of hemolytic patterns
23
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What is the classification of Chocolate Agar?
Enriched
24
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What is the inhibitor in Chocolate Agar?
None
25
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What makes Chocolate Agar different from Blood Agar?
Blood is lysed to release hemoglobin and X & V factors
26
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What is the use of Chocolate Agar?
Cultivation of fastidious organisms like Neisseria spp., Brucella spp., and Haemophilus spp.
27
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What is the classification of Thiosulfate-Citrate-Bile Salts (TCBS) Agar?
Selective and Differential
28
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What is the indicator in TCBS Agar?
Bromthymol Blue
29
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What inhibits Gram-positive bacteria in TCBS Agar?
1% NaCl and Bile Salts
30
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What inhibits intestinal flora in TCBS Agar?
Increased pH
31
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What are other components of TCBS Agar?
Citrate, Sucrose, Ferric Citrate
32
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What does a yellow colony on TCBS Agar indicate?
Fermenter
33
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What does a green colony on TCBS Agar indicate?
Nonfermenter
34
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What is the use of TCBS Agar?
Isolation of Vibrio spp.
35
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What is the classification of Salmonella-Shigella (SS) Agar?
Selective and Differential
36
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What is the pH indicator in SS Agar?
Neutral Red
37
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What inhibits intestinal flora in SS Agar?
Brilliant green and bile salts
38
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What are the other components of SS Agar?
Lactose, Ferric citrate, Sodium citrate
39
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What is the colony appearance of Shigella spp. on SS Agar?
Colorless
40
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What is the colony appearance of Salmonella spp. on SS Agar?
Colorless with black center (H₂S production)
41
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What is the use of SS Agar?
Isolation of Salmonella and Shigella spp.
42
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What is the classification of Hektoen Enteric (HE) Agar?
Selective and Differential
43
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What is the pH indicator in HE Agar?
Bromthymol Blue
44
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What are the inhibitors in HE Agar?
Bromthymol Blue and Acid Fuchsin
45
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What are the other components of HE Agar?
Bile salts, Lactose, Sucrose, Salicin, Ferric ammonium citrate
46
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What is the colony appearance of nonenteric organisms on HE Agar?
Orange to Salmon colonies
47
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What is the colony appearance of Salmonella/Shigella on HE Agar?
Blue-green colonies
48
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What is the use of HE Agar?
Isolation of Salmonella and Shigella spp.
49
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What is the classification of Xylose-Lysine-Deoxycholate (XLD) Agar?
Selective and Differential
50
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What is the pH indicator in XLD Agar?
Phenol Red
51
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What is the inhibitor in XLD Agar?
Sodium deoxycholate
52
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What are the other components of XLD Agar?
Xylose, Lactose, Sucrose, Ferric ammonium citrate
53
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What is the colony appearance of fermenters on XLD Agar?
Yellow
54
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What is the colony appearance of Shigella on XLD Agar?
Red (nonxylose fermenter)
55
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What is the colony appearance of Salmonella on XLD Agar?
Red with black center (H₂S production)
56
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What is the use of XLD Agar?
Isolation of Salmonella and Shigella spp.
57
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What is the classification of Mannitol Salt Agar (MSA)?
Selective and Differential
58
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What is the pH indicator in MSA?
Phenol Red
59
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What is the inhibitor in MSA?
7.5% NaCl
60
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What is the other component of MSA?
Mannitol
61
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What is the appearance of pathogenic Staphylococcus on MSA?
Yellow agar
62
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What is the appearance of nonpathogenic Staphylococcus on MSA?
No color change
63
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What is the use of MSA?
Isolation of Staphylococcus spp.
64
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What is the classification of Thayer Martin Agar (TMA)?
Selective
65
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What are the inhibitors in TMA?
Vancomycin, Colistin, Nystatin
66
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What is the base medium used in TMA?
Chocolate Agar
67
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What is the use of Thayer Martin Agar?
Isolation of Neisseria spp.
68
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What is the classification of Modified Thayer Martin Agar?
Selective
69
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What additional inhibitor is present in Modified Thayer Martin Agar compared to TMA?
Trimethoprim lactate
70
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What is the base medium used in Modified Thayer Martin Agar?
Chocolate Agar
71
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What is the use of Modified Thayer Martin Agar?
For Neisseria spp.
72
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What is the classification of Martin Lewis Medium?
Selective
73
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What are the inhibitors in Martin Lewis Medium?
Vancomycin, Colistin, Anisomycin, Trimethoprim Lactate
74
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What is the base medium used in Martin Lewis Medium?
Chocolate Agar
75
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What is the use of Martin Lewis Medium?
For Neisseria spp.
76
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What is the classification of New York City Agar?
Selective
77
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What are the inhibitors in New York City Agar?
Vancomycin, Colistin, Trimethoprim Lactate, Amphotericin B
78
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What is the base medium used in New York City Agar?
Chocolate Agar
79
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What is the use of New York City Agar?
For Neisseria spp.
80
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What other organisms may also grow on New York City Agar?
Mycoplasma hominis and Ureaplasma urealyticum
81
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What is the classification of CIN (Cefsulodin-Irgasan-Novobiocin) Agar?
Selective
82
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What are the indicators in CIN Agar?
Neutral Red and Crystal Violet
83
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What are the inhibitors in CIN Agar?
Cefsulodin, Irgasan, Novobiocin
84
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What are the other components of CIN Agar?
Mannitol, Bile salts
85
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What is the colony appearance of Yersinia spp. on CIN Agar?
Colorless with red center (bull’s eye)
86
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What is the use of CIN Agar?
For Yersinia spp. (Aeromonas spp. may also grow)
87
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What is the classification of Bordet-Gengou Agar?
Selective
88
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What is the inhibitor in Bordet-Gengou Agar?
Methicillin or Cephalexin
89
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What are the other components of Bordet-Gengou Agar?
15–20% defibrinated blood, potato infusion
90
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What is the colony appearance on Bordet-Gengou Agar?
Beta hemolysis
91
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What is the use of Bordet-Gengou Agar?
For Bordetella pertussis
92
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What is the classification of Columbia CNA Agar?
Selective
93
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What are the inhibitors in Columbia CNA Agar?
Nalidixic Acid and Colistin
94
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What is the base medium used in Columbia CNA Agar?
Columbia agar base
95
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What is the use of Columbia CNA Agar?
For Gram (+) bacteria
96
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What is the classification of Loeffler Medium?
Enriched, Selective
97
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What are the components of Loeffler Medium?
Horse serum, beef extract, dextrose, proteose peptone, sodium chloride
98
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What is the colony appearance on Loeffler Medium?
Small, cream-colored colonies with raised centers; metachromatic granules when stained with methylene blue
99
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What is the use of Loeffler Medium?
For Corynebacterium spp.
100
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What is the classification of Brain-Heart Infusion Agar?
General Purpose
Note 
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