Biochem - Exam #1

Ch. 1-5

Order the interactions in terms of overall bond strength from weakest to strongest

Van der Waals, Hydrogen Bonds, Covalent Bonds

All of the following characteristics are found In Eukaryotic Cells EXCEPT:

Circular Chromosomes

What is the reason for existence of the hydrophobic effect?

Tendency of nonpolar molecules to self-associate in presence of an aqueous solution

What would the concentration of hydroxide (OH^-) ions in a solution with a pH of 6?

10^-8

If a 0.3 M solution has a pH of a 6.5, and the pKa of the acid in solution is 6.5, what would be the concentration of the dissociated [H⁺] in the solution?

0.15

Given the following different buffers, which would be the most effective near pH of 7.0?

MOPS (3-N-morpholino)propanesulfonic acid), pKa = 7.15

Which of the following chemicals could be considered a weak acid?

Acetic Acid

Dodecanoic acid, found in coconut oil and some hand soaps, would be an example of which type of organic molecule?

Carboxylic Acid

A primary alcohol is condensed with carboxylic acid in a reaction. This will form a/an:

Ester

With Henderson-Hasselbalch equation, what would the pH of a buffer solution made from 0.25 M CH₃COOH and 0.05 M CH₃COO^- which has a pKa = 4.7?

4.0

What is the basis for the hydrophobic effect?

The tendency of non-polar molecules to self-associate in the presence of an aqueous solution

What is the concentration of hydroxide ions [OH^-] in an aqueous solution with pH = 9?

10^-5 M

What is the amino-terminal residue in the sequence of amino acid Gly-Tyr-Gly-Phe-Leu?

Glycine

What is the level of protein structure which concerns the overall three-dimensional shape of a multiple subunit protein?

Quaternary

Which amino acid is represented by the letter Q?

Glutamine

Which amino acid is represented by the following structure?

Tryptophan

What pair of amino acids is positively charged at a neutral pH?

Lys, Arg

What would be the most likely problem for a patient if prolines are not hydroxylated in post-translational modification?

Osteogenesis imperfecta

What method can be used to separate specific proteins by their molecular weight from a mixture of proteins?

Affinity chromatography

The FIRST step in protein purification from a homogenate of cellular organelles is:

Centrifugation

Which technique allows you to identify a protein with an antibody after electrophoresis?

Western Blog