types of media

CULTURE MEDIA

Any medium that contains all what is needed to support bacterial growth

CULTURE

refers to Growth of organism in culture media

Pure culture

Types of Culture with only 1 species

mixed culture

Types of Culture with more than 1 species

stock culture

Types of Culture used in quality control procedures (i.e. ATCC-American Type Culture Collection)

General Purpose

Contains only what is needed to support bacterial growth

Nutritive media

For routine cultivation, examples: nutrient agar/broth, BHI, TSB (trypticase soy broth)

Enrichment media

To enhance bacterial growth, to increase bacterial yield

salmonella

Selenite broth __________________________

Tetrathionate broth ______________________

vibrio

APW-alkaline Peptone water _______________

Salmonella

Buffered Peptone water

Enriched media

For growing fastidious organisms

neisseria and haemophilus

Fastidious organisms that can grow with enriched media are

Sheep’s blood

In the preparation of BAP most preferred

Human blood

In the preparation of BAP least preferred

Streptococci

Sheep’s Blood

Haemphilus

Horse Blood

G. vaginalis

Human Blood

Trypticase Soy Broth

for subculturing various bacteria from primary agar plates

enriched, differential

BLOOD AGAR is classified as __________

lysed

Reason why CAP cannot be used to detect hemolysis is because RBC already ____

Selective media

Used to promote growth of a particular organism & at the same time preventing growth of other. It contains inhibitor

campylobacter spp

Selective media Campy-Blood Agar, Skirrow’s are for

Yersinia enterocolitica

Selective media CIN (Cefsulodin Irgasan Novobiocin)

clostridium difficile

Selective media Cycloserine cefoxitin Fructose agar (CCFA)

listeria monocytogenes

Selective media Mac Bride Medium

Neisseria

Selective media thayer martin

Differential Media

to differentiate organisms that are growing together, allows bacteria to be visually differentiate

EMB, Mcconkey

Differential Media examples _____, ______

Mcconkey

_______ differentiates lactose (pink) to non lactose (colorless) fermenter

Vibrio; salt

Selective & Differential TCBS _______ Inhibitor increase ________ concentration

Sucrose; bromthymol blue

Selective & Differential Fermentable CHO _______ indicator _______

green

Selective & Differential TCBS uninocculated color

yellow

Selective & Differential sucrose fermenter color for v. cholorae

Transport Media

to maintain viability of organism during specimen transport

neisseria

Transport Media JEMBEC and transgrow

Vibrio

Transport Media Cary Blair for stool pathogen

Biochemical test media

Used for biochemical testing which aids in bacterial identification

Carb (CHO)

Biochemical test media TSI _____ fermentation test

lysine iron agar

Biochemical test media LIA

citrine

Biochemical test media Simmon Citrate Agar _______ utilization test

urease test

Biochemical test media Urea agar

Mueller Hinton Agar

Media for susceptibility test routine

middlebrook

Media for susceptibility test suited for Mycobacterium spp only

Autoclaving

Media Sterilization

121, 15, 15-30

In autoclaving it should be ____ degC, ____ psi, ___ - ___ mins

Blood Cystine Dextrose Agar

For growing Francisella tularensis

Bordet-Gengou Media

Isolation of Bordetella pertussis

Brilliant Green Agar (BGA)

Selective media for isolation of Salmonella other than S. typhi.

Buffered Charcoal Yeast Extract Agar with Antibiotics

Enrichment and selective for Legionella spp.

Chapman Stone agar

Selective media for the isolation of Staphylococcus

Campylobacter Thioglycollate Broth

Selective holding medium for recovery of Campylobacter spp.

Casein Media

Used for separating Nocardia & Streptomyces. Made from skimmed milk. Used as a media to test for hydrolysis

Cefoperazone, Vancomycin, Amphotericin (CVA)

Selective media for isolation of Campylobacter spp

Chopped Meat Glucose

Used for primary isolation of anaerobes. Used for Clostridium spp.

Cystine Trypticase Agar (CTA)

with carbohydrates added is excellent for determining the fermentation reactions of groups such as Neisseria

Dieudonnes media

Selective media for Vibrios

“E” agar and Edward Hayflick agar

Selective media for the isolation of Mycoplasma and Ureplasma

Egg Yolk Agar

Used for the isolation and identification of Clostridium

Fetal Bovine serum with Vancomycin

Selective media for H. ducreyi

Fildes Enrichment

Used for isolation of H. influenza

Granada Medium

Selective for S. agalactiae

Kelly’s medium

Borrelia spp

For Bacillus cereus

MEYP (Mannitol Egg Yolk Polymyxin B agar)

PEMBA (Polymyxin Egg Yolk Mannitol Bromthymol Blue agar)

Modified Kelly’s medium or Barbour Stoenner Kelly

Selective media for the isolation of Borrelia species

Regan Lowe/ Charcoal Cephalexin Agar

Enrichment and selective media for Bordetella pertussis

Schaedler Agar

Non-selective media for the recovery of anaerobes and aerobes

Mycoplasma

Shepard’s medium (SP4), A7B

TODD HEWITT BROTH

To detect genital carriage of S. agalactiae during pregnancy

LIM BROTH

Modified Todd Hewitt Broth, contains colistin and nalidixic acid

Tap water agar

to differentiate AEROBIC ACTINOMYCETES