AEROBIC GRAM + BACILLI

Corynebacterium

Rothia

Listeria

Non spore forming

Non branching

Catalase (+) Bacilli

Corynebacterium

club shaped

Kleb Loefflers bacillus

Corynebacterium also known as_______

Erysipelothrix

only H2S (+) in aerobic gram (+) bacilli

Diphtheria toxin

major virulence factor of Corynebacterium

Fragment A

fragment of Diphtheria toxin that produce cytotoxicity (destruction of the cell)

Fragment B

fragment of Diphtheria toxin that is the attachment to the host of the cell

Corynebacterium diphtheriae

bull’s neck appearance

respiratory diphtheria

cutaneous diphtheria

2 clinical infections of Corynebacterium diphtheriae

Corynebacterium

highly pleomorphic gram (+) bacillus

Babe’s Ernst granules

metachromatic granules of Corynebacterium diphtheriae

Loeffler serum

Pai agars

agars that enhance of metachromatic granules

Cystine - tellurite blood agar

selective differential medium of Corynebacterium diphtheriae

C. diphtheriae

C. ulcerans

C. pseudotuberculosis

diphtheria toxin (+)

Urease test

test that differentiate:

C. diphtheriae

C. ulcerans

C. pseudotuberculosis

C. diphtheriae

-urease (-)

-ferments glucose and maltose

-producing acid, but not gas

-reduces nitrate to nitrate

ELEK test

immunodiffusion test
(+) lines of precipitation

Penicillin

drug of choice of C. diphtheriae

Erythromycin

used for penicillin sensitive individuals

C. amycolatum

flat and dry, have a matte or waxy appearance, and are non lipophilic

C. jeikeium

-named after Johnson and Kaye

-lipophilic

C. jeikeium

most commmon cause of Corynebacterium-associated prosthetic valve endocarditis in adults

C. pseudotuberculosis

-causes granulomatous lymphadenitis

-primarily a veterinary pathogen

C. pseudotuberculosis

-dermonecrotic toxin

-produces brown halo on CTBA

C. striatum

-nonliphophilic and pleomorphic

-produces small shiny convex colonies in 24 hrs

-susceptible to vancomycin

C. ulcerans

-produces a brown halo around colonies on CTBA

-grow well on SBA and produces a narrow zone of b-hemolysis

C. urealyticum

-most commonly associated with UTIs

-lipophilic

-pinpoints, non hemolytic, white colonies

-drug on choice: vancomycin

Rothia mucilaginosa

linked to bacteremia,endocarditis, pneumonia

Rothia dentocariosa

member of the normal human oropharyngeal flora and found in saliva

Rothia

-resembles coryneform bacilli

-produces coccoid cells

Listeriosis

is recognized as an uncommon but serious infections primarily of neonates, pregnant women, older adults, and immunocompromised hosts

McBride medium

selective differential medium of Listeria monocytogenes

30C - 35C

optimal growth temp

Listeria monocytogenes

-hippurate hydrolysis (+) like S. agalactiae

-catalase (+)

-bile esculin hydrolysis (+)

-motile

tumbling motility

umbrella pattern

motility of Listeria monocytogenes

25C

motility temperature of Listeria monocytogenes

Erysipelothrix rhusiopathiae

-only can infect human

-thin, rod-shaped

-gram variable

Erysipeloid

-butchers cut

-fish vendors

-whale finger

-seal finger

test tube brush

gelatin stab culture of Erysipelothrix rhusiopathiae at 22C

Arcanobacterium haemolyticum

-formerly Corynebacterium haemolyticum

-catalase (-)

-small colonies on SBA

Arcanobacterium haemolyticum

-pitting of the agar

-lipase and lecithinase (+)

-exhibits reverse CAMP reaction (hemolysis -)

Gardnerella vaginalis

-clue cells

-grows best in 5% - 7% CO2

-35C - 37C

Human Blood bilayer tween agar

selective medium of Gardnerella vaginalis

Actinomadura

causes mycetomas

nocobactin

ironchelating compoung of nocardia

Modified Thayer Martin Agar
Buffered Charcoal - Yeast extract agar

Medium of Nocardia

Tropheryma whipplei

whipple disease

protective antigen

EF

LF

3 protein of anthrax toxin

Cutaneous Anthrax

occur when wounds are contaminated with anthrax spores

Eschar

black eschar

malignant pustule

other names of cutaneous anthrax

inhalation anthrax

woolsorter’s diseasein

injectional anthrax

characterized ny soft tissue infection associated with skin popping or other forms of injection

medusa head

beaten egg whites

cut glass appearance

Bacillus anthracis