microbio- Enterobacteriaceae

rods

what is the shape of family Enterobacteriaceae?

negative; positive

bacteria in the family Enterobacteriaceae are oxidase _____ and catalase _____

yes, they have perithrichous flagella

are family Enterobacteriaceae motile bacteria?

peritrichous

family Enterobacteriaceae have ________ flagella

Macconkey, nonenriched, BG agar, Levine agar, ASAP, XLD

on what type of cultural media can family Enterobacteriaceae grow?

both- they are facultatively anaerobic

do family Enterobacteriaceae grow with or without oxygen?

Escherichia coli

Salmonella

Yersinia

Proteus

Enterobacter

Klebsiella

what 6 genera do we study in family Enterobacteriaceae?

rod; peritrichous flagella

family Enterobacteriaceae look like this. what shape and type of flagella do they have?

Enteropathogenic (EPEC)

Enterotoxigenic (ETEC)

Enteroinvasive (EIEC)

Enterohemorrhagic (EHEC)

Enteroaggregative (EAST1)

Attaching and effacing (AEEC)

what are the 6 stains of E. coli?

it adheres to the enterocytes and eliminates microvilli

how does enteropathogenic E. coli work?

no, they eliminate the microvilli without producing toxins

do enteropathogenic E. coli produce enterotoxins to harm the enterocytes?

watery diarrhea

what type of diarrhea is associated with enteropathogenic E. coli?

E. coli

it is lactose +

which enterobacteria produces these colonies on macconkey agar?

E. coli

which enterobacteria has lactose, which produces pink colonies on Macconkey agar?

E. coli

on BC agar, which genus of the family Enterobacteriaceae produces these yellow colonies?

E. coli

which genus of the family Enterobacteriaceae produces metallic colonies on levine agar?

mastitis

reproductive infections

septicemia

what extraintestinal infections can E. coli occasionally produce?

+

is E. coli indole + or -?

A (+)

which indole result does E. coli produce?

B (-)

which indole result does Salmonella produce?

it has enterotoxins that affect the function of enterocytes in neonatal and weaned piglets, calves, and lambs

how does Enterotoxic E. coli work pathogenically?

Enterotoxic E. coli (ETEC)

what strains of E. coli does this describe: it's enterotoxins affect the function of enterocytes in neonatal and weaned piglets, calves, and lambs

it invades the enterocyte and multiplies inside of it

how does enteroinvasive E. coli work pathogenically?

enteroinvasive E. coli (EIEC)

which E. coli strain invades the enterocyte and multiplies within it?

enterohemorrhagic E. coli (EHEC)

which E. coli strain has verotoxins/shiga toxins?

it has verotoxins/shiga toxins that destroys enterocytes and causes septicemia

how does enterohemorrhagic E. coli work?

ruminants

the main reservoir species of enterohemorrhagic E. coli is _________

enterohemorrhagic E. coli (EHEC)

what E. coli strain most commonly produces septicemia?

Enterohemorrhagic E. coli (EHEC)

what type of E. coli produces bloody diarrhea?

bloody diarrhea

what type of diarrhea does enterohemorrhagic E. coli cause?

it produces an aggregation of E. coli, eliminates microvilli, and produces enterotoxins

how does enteroaggregative E. coli work pathogenically?

yes

does enteroaggregative E. coli produce enterotoxins?

watery mucoid diarrhea, sometimes with traces of blood

what type of diarrhea does enteroaggregative E. coli cause?

it adheres to enterocytes and causes elongation of their microvilli

how does attaching and effacing E. coli work?

enteropathogenic E. coli (EPEC)

enteroinvasive E. coli (EIEC)

which strain of E. coli produce watery diarrhea?

because it can cause septicemia

this is most common with enterohemorrhagic E. coli

why can E. coli cause pulmonary fibrosis? which strain is most likely to cause this?

Attaching and effacing E. coli (AEEC)

which E. coli strain causes elongation of the microvilli on enterocytes?

E. coli

because it ferments both lactose and glucose

which genus of family Enterobacteriaceae produces this result in a TSI test?

lactose and glucose

what carbohydrates does E. coli ferment?

Salmonella Typhimurium

Salmonella Enteriditis

what are the 2 serotypes in the genus Salmonella?

fecal-oral route, usually feces with Salmonella contaminates food/water that is then ingested

how does salmonella enter the body?

it attaches to enterocytes and penetrates them, and then replicate in their vesicles and become engulfed by macrophages

after the entrance of Salmonella via ingestion, what does it do?

yes

can Salmonella produce septicemia?

Salmonella

which genus of family Enterobacteriaceae commonly causes subclinical infections, where the bacteria remains latent in the lymph nodes, but can be activated by stress?

stress; acute fatal septicemia

if Salmonella becomes latent in the lymph nodes of its host, it can be triggered by _____ and cause ______

yes, it has LPS, an endotoxin that causes a local inflammatory response, leading to damage to intestinal epithelial cells

does Salmonella have toxins?

diarrhea

food poisoning

septicemia

abortion

what are the clinical signs of Salmonella?

Salmonella

which bacteria belonging to family Enterobacteriaceae produces pink colonies on brilliant green (BG) agar?

yellow

which type of reaction does Salmonella produce on Macconkey agar?

Salmonella- it is lactose -

which enterobacteria causes this result on Macconkey agar?

yes

can all bacteria in the family Enterobacteriaceae grow on macconkey agar?

-; produces a yellow macconkey

is Salmonella lactose + or -? what reaction does this produce on macconkey agar?

+; produces a pink macconkey

is E. coli lactose + or -? what reaction does this produce on macconkey agar?

Salmonella

which Enterobaceriaceae produces black colonies on XLD agar?

Salmonella

the purple colonies on this ASAP agar plate are what bacteria?

-

is Salmonella indole + or -?

the left 2 tubes- black

because it produces H2S and ferments glucose

which TSI test does Salmonella produce? why?

yes, all bacteria in the family Enterobacteriaceae ferments glucose

does Salmonella ferment glucose?

Salmonella- it both ferments glucose and produces H2S

which genus in family Enterobacteriaceae produces this TSI result?

Y. pestis

Y. pseudotuberculosis

Y. enterocolitica

what 3 species do we study in the genus Yersinia?

flea and rat bites, sometimes cat bites and scratches

how is Yersinia pestis transmitted?

bubonic plague

pneumonic plague

which well-known diseases are caused by Yersinia pestis?

Yersinia pestis

what bacteria causes bubonic plague and pneumonic plague?

Yersinia- it only ferments glucose

what bacteria in the family Enterobacteriaceae produces this result with a TSI test?

Yersinia pseudotuberculosis

(acute necrotizing enteritis is a type of pseudotuberculosis)

which enterobacteria causes acute necrotizing enteritis in wild rodents, lagomorphs, and birds?

Yersinia pseudotuberculosis

what enterobacteria causes these granulomas?

ingestion

how is Yersinia pseudotuberculosis transmitted?

ingestion

how is Yersinia enterocolitica transmitted?

Yersinia pestis

which enterobacteria is transmitted by flea, rat, and cat bites and scratches?

acute necrotizing enteritis/pseudotuberculosis

wild rodents, lagomorphs, and birds

what is the clinical disease of Yersinia pseudotuberculosis and which animals does it more commonly effect?

no

does Yersinia ferment lactose?

Y. enterocolitis

what Yersinia species causes a latent infection, with ability to become acute gastroenteritis?

Y. pestis

which is the most invasive species of Yersinia?

it has an antiphagocytic protein capsule and a plasminogen activator that helps systemic spread

what factors make Yersinia pestis so invasive?

it can do both, it is facultatively intracellular

does the bacteria Yersinia replicate inside or outside of cells?

mesenteric lymph nodes

Yersinia is engulfed by macrophages and transported to ________

P. mirabilis

P. vulgaris

what are the 2 important species of the genus Proteus?

false- these species rarely cause enteric disease in domestic animals, they are normal microbiota of the animal's GI tract and can sometimes, but rarely, cause localized infection

true or false: Proteus mirabilis, Proteus vulgaris, Enterobacter aerogenes, and Klebsiella pneumoniae are all enterobacteria that are a common cause of enteric disease in domestic animals

Enterobacter

Proteus

Klebsiella

which bacteria in the family Enterobacteriaceae are opportunistic pathogens- normally living in the GI tract of animals, but certain situations can cause overgrowth, which will lead to a localized infection?

coliform mastitis in cows and sows

what clinical disease can be caused by Enterobacter aerogenes?

Enterobacter aerogenes

which bacteria in the family Enterobacteriaceae can cause coliform mastitis in cows and sows when conditions cause its overgrowth?

Proteus mirabilis and vulgaris

what bacteria in the family Enterobacteriaceae has the potential to cause UTIs in dogs and horses and otitis externa in dogs?

Proteus mirabilis/vulgaris

which enterobacteria can cause otitis externa in dogs?

intercurrent infection

tissue devitalization

inherent vulnerability of certain organs

what factors predispose an animal for clinical disease by Enterobacter, Proteus, or Klebsiella?

a capsular material that inhibits phagocytosis

adhesins that allow attachment (especially in the urinary tract)

LPS- causes inflammatory response, which leads to pyrexia, endothelial damage, and microthrombosis

what are the pathogenic factors that Proteus

Enterobacter and

Klebsiella have?

urinary tract

Proteus, Enterobacter, and Klebsiella have adhesins that allow attachment to host cells, especially in what part of the body?