Experiment 08: Enzymes

Enzymes

These are complex organic molecules with definite chemical structure that hastens chemical reaction without getting consumed in the process

concentration of substrate, concentration of enzymes, concentration of the products of reaction, pH, temperature, inorganic salts, presence of inhibitors and activators

Factors that affect enzyme activity

oxidases, catalase, peroxidase

What are the 3 types of enzymes?

Oxidase

catalyzes redox reactions and uses oxygen as electron acceptor

Catalase

catalyzes decomposition of H2O2 into H2O and O2

Peroxidase

catalyzes oxidation of hydrogen donor with the help of peroxides

turned brown

Positive result for oxidase on fruit

melanin

The exposed are of the fruit turned brown because of the formation of _____________________

Tyrosinase

Example of oxidase in fruit

Potato

Main source of catalase

H2O2

Oxidizing and bleaching agent for catalase

H2O2

Substrate of catalase

Unboiled, because the enzyme catalase has not yet been denatured

Which has more bubbles boiled or unboiled filtrate? Why?

Monophenol oxidase, Polyphenol oxidase, Cytochrome oxidase

3 types of oxidases in potato

Monophenol oxidase

Responsible for oxidizing phenol to cathecol

Polyphenol oxidase

Responsible for oxidizing cathecol to O-quinone

Cytochrome oxidase

Oxidizes phenyldiamine to form indophenol

a. light pink

b. light orange

b, brown

Oxidases from Potato results:

a. phenol

b. cathetcol

c. pyrogallol

a. light pink

b. light orange

c. brown

Peroxidase from Potato:

a. phenol

b. cathecol

c. pyrogallol

continuous glow of splint

Positive result for catalase in liver