Chp.2 Urine & BF Automation

What determines the result in urinalysis automation and what is the most important when determining? (2)

• Color

• Accuracy of the timing

What is the ultimate goal of the urine analysis?

Improve reproducibility and color discrimination while increasing productivity and standardization for reporting results

Automated reagent strip readers use a spectrophotometric measurement of ___________

Reflectance photometry

Semi-Automated Analyzers (lab size, calibration, abnormal result, checks)

• Small and medium-volume size

• Self-calibrating

• Abnormal result are flagged and stored sent to the LIS

• Auto-check

Fully-Automated Analyzers (lab size, abnormal result)

• High-volume laboratory

• Abnormal ranges to be selected so confirmatory testing and sent to the LIS

Advantge of Microscopy Analyzers (3)

• Cost-effective

• Improve TAT’s

• Flow cytometry and digital imaging

Sysmex UF-1000 Fully Automated (methodology, urine, result, others)

• Flow cytometry

• 1.2 mL of uncentrifuged urine

• Results in quantitative cells per microliter and cells per high- or low-powered field

• Independently or integrated with an automated urine chem strip

Sysmex UN-2000 Automated (methodology, core, surface, result)

• Urine cytometry with digital image

• Core channel: nuclear materials

• Surface channel: anucleate elements

• result reporting and reflex rule setting

Beckman’s Coulter’s iQ200 Microscopy (methodology, urine, result, others)

• digital flow morphology and particle recognition

• 1.3 mL of urine or bf

• results are reviewed or auto-released to the LIS

Classification categories in Beckman’s Coulter’s iQ200 Microscopy (5)

• RBC’s, WBC’s, WBC clump

• Epithelial cells

• Casts

• Crystals

• Bacteria, yeast, mucus, and sperm

Electonika UriSed 2 and 3 Pro (methodology, urine, others)

• automated microscopy with digital imaging

• 2mL of urine

• 3 Pro imporves in differentiation of elements

FUS-100 and FUS-200 (methodology, others)

• Flat-flow cell digital imaging and neural network

• Classifies 12 visible components based on shape, contrast, texture, and frequency

Clinitek AUWi System (methodology, urine, result, others)

• Automated urinalysis

• 5mL urine

• Results are verified automatically

• No centifugation is required

iRICELL (methodology, urine, result)

• urine chem and microscopy analyzer

• 4 mL urine

• Results transmitted to LIS

LaUMat 2 with UriSed 2 and 3 (methodology, urine)

• physical and chemical tests

• 4mL urine

COBAS 6500 (methodology, urine, others)

• Urine chemistry analyzer

• 2.8mL urine

• Particle recognition software determines the identification of cells

UX-2000 (methodology, urine, others)

• flow cytometry

• 5mL urine

• Physicial examination + microscopic examination

How to do body fluid analysis automation

Hemocytometer for WBC differentials

Advantages of BF analysis automation (3)

• Precision

• Faster TATs

• Standardization of results

ADVIA 2120i (methodology, cell count results)

• Flow cytometry, light scattering, and absorbance to count RBCs and WBC

• WBC differential available

Sysmex XN-series (methodology, cell count results, others)

• Enumerations and flow cytometry

• Reports BF WBCs and total nucleated cell counts

• Identifies Malignant tumor cells

Beckman Coulter’s iQ200 (cell count results)

• Total cells, nucleated cells, and RBCs