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Chemical analysis
The process of analyzing the chemical composition of a substance or sample.
Acid-soluble pool
The fraction of organic compounds that can be dissolved in acid.
Acid-insoluble fraction
The fraction of organic compounds that cannot be dissolved in acid.
Biomolecules
Carbon compounds found in living tissues.
Elemental analysis
The analysis of the elemental composition of a substance or sample.
Wet weight
The weight of a living tissue sample before it is dried.
Dry weight
The weight of a living tissue sample after all the water has evaporated.
Ash
The remaining material after a living tissue sample has been fully burnt.
Inorganic elements
Elements that are not carbon-based and are found in living organisms.
Inorganic compounds
Compounds that are not carbon-based and are found in living organisms.1. Amino acids:Organic compounds that are the building blocks of proteins.
R group
The variable group in an amino acid that determines its specific properties.
Glycine
An amino acid with a hydrogen R group.
Alanine
An amino acid with a methyl R group.
Serine
An amino acid with a hydroxy methyl R group.
Acidic amino acids
Amino acids with both amino and carboxyl groups, giving them an acidic property.
Basic amino acids
Amino acids with both amino and carboxyl groups, giving them a basic property.
Neutral amino acids
Amino acids with both amino and carboxyl groups, neither acidic nor basic.
Aromatic amino acids
Amino acids with a ring structure, such as tyrosine, phenylalanine, and tryptophan.
Zwitterionic form
The ionizable form of an amino acid in solution, with both positive and negative charges.
Lipids
Organic compounds that are generally water insoluble, including fatty acids and glycerol.
Fatty acids
Organic compounds with a carboxyl group attached to an R group, can be saturated or unsaturated.
Glycerol
A trihydroxy propane compound found in lipids.
Triglycerides
Lipids composed of glycerol and three fatty acids.
Phospholipids
Lipids with phosphorous and a phosphorylated organic compound, found in cell membranes.
Nucleotides
Organic compounds composed of a sugar, nitrogen base, and phosphate group, found in nucleic acids.
Nucleosides
Organic compounds composed of a sugar and nitrogen base, without the phosphate group.
DNA
Deoxyribonucleic acid, a nucleic acid that carries genetic information.
RNA
Ribonucleic acid, a nucleic acid involved in protein synthesis.
Primary metabolites
Compounds found in animal tissues, including amino acids and sugars.
Secondary metabolites
Compounds found in plant, fungal, and microbial cells, including alkaloids, flavonoids, and essential oils.
Biomacromolecules
Macromolecules found in the acid insoluble fraction of living organisms, including proteins, nucleic acids, polysaccharides, and lipids.1. Biomolecules:The molecules that make up living organisms, including water, proteins, carbohydrates, lipids, nucleic acids, and ions.
Water
The most abundant component of cells, making up 70-90% of the total cellular mass.
Proteins
Polypeptides made up of linear chains of amino acids linked by peptide bonds. They have various functions in living organisms, such as transporting nutrients, fighting infectious agents, acting as hormones, and serving as enzymes.
Carbohydrates
Polysaccharides that are long chains of sugars. They serve as a source of energy and include molecules like cellulose, starch, and glycogen.
Lipids
Organic compounds that are not strictly macromolecules. They are present in cell membranes and other membranes, and can form vesicles that are not water soluble.
Nucleic acids
Macromolecules that include DNA and RNA, which are responsible for storing and transmitting genetic information.
Ions
Charged particles that are present in cells and play important roles in various cellular processes.
Cell membrane
The structure that surrounds and protects the cell, composed of lipids and proteins.
Vesicles
Small membrane-bound sacs that can form from the fragmentation of cell membranes and other membranes.
Acid insoluble pool
The fraction of macromolecules that are not soluble in acid, including membrane fragments and vesicles.
Acid soluble pool
The fraction of macromolecules that are soluble in acid, representing the cytoplasmic composition of cells.
Cytoplasm
The fluid inside cells, where various cellular processes take place.
Organelles
Specialized structures within cells that perform specific functions.
Polymer
A large molecule made up of repeating subunits called monomers.
Amino acids
The building blocks of proteins, of which there are 20 different types.
Peptide bonds
The chemical bonds that link amino acids together to form proteins.
Heteropolymer
A polymer that consists of different types of monomers.
Homopolymer
A polymer that consists of only one type of monomer repeating multiple times.
Essential amino acids
Amino acids that our body cannot produce and must be obtained through our diet.
Non-essential amino acids
Amino acids that our body can produce on its own.
Collagen
The most abundant protein in the animal world, which provides structural support to tissues.
Enzyme
A protein that catalyzes chemical reactions in living organisms.
Hormone
A protein that acts as a chemical messenger in the body, regulating various physiological processes.
Antibody
A protein that fights against infectious agents, such as bacteria and viruses.
Receptor
A protein that detects and responds to specific molecules, such as hormones or neurotransmitters.
Glucose transporter 4 (GLUT-4)
A protein that enables the transport of glucose into cells.
Polysaccharides
Long chains of sugars that serve as another class of macromolecules, including cellulose, starch, and glycogen.
Cellulose
A polysaccharide made up of glucose monomers, providing structural support to plant cell walls.
Starch
A polysaccharide that serves as a storage form of energy in plant tissues.
Glycogen
A polysaccharide that serves as a storage form of energy in animals.
Inulin
A polysaccharide made up of fructose monomers.
Reducing end
The right end of a polysaccharide chain, which can undergo chemical reactions.
Non-reducing end
The left end of a polysaccharide chain, which does not undergo chemical reactions.
Helical structure
A spiral-shaped secondary structure, such as the helical portion of starch.
I2
Iodine molecules, which can be held by starch in its helical portion, resulting in a blue color.1. Cellulose:A complex polysaccharide found in plant cell walls and used to make paper and cotton fiber.
Chitin
A complex polysaccharide found in the exoskeletons of arthropods.
Nucleic acids
Macromolecules found in the acid insoluble fraction of living tissues, composed of polynucleotides.
Nucleotide
The building block of nucleic acids, consisting of a heterocyclic compound, a monosaccharide, and a phosphoric acid or phosphate.
Adenine
A nitrogenous base found in nucleic acids, specifically a substituted purine.
Guanine
A nitrogenous base found in nucleic acids, specifically a substituted purine.
Uracil
A nitrogenous base found in RNA, specifically a substituted pyrimidine.
Cytosine
A nitrogenous base found in nucleic acids, specifically a substituted pyrimidine.
Thymine
A nitrogenous base found in DNA, specifically a substituted pyrimidine.
Primary structure
The sequence of amino acids in a protein.
Secondary structure
The folding of a protein into helices or other forms.
Tertiary structure
The overall 3-dimensional shape of a protein.
Quaternary structure
The arrangement of multiple polypeptides or subunits in a protein.
Enzymes
Proteins that catalyze biochemical reactions.
Ribozymes
Nucleic acids that behave like enzymes.1. Biomolecules:Molecules that are essential for life, including proteins, carbohydrates, lipids, and nucleic acids.
Active site
A crevice or pocket on an enzyme where the substrate binds and the catalytic reaction takes place.
Enzyme
A protein with a three-dimensional structure that acts as a catalyst, speeding up chemical reactions in living organisms.
Inorganic catalysts
Catalysts that work efficiently at high temperatures and pressures.
Organic catalysts
Catalysts, such as enzymes, that are derived from living organisms and have specific temperature and pH requirements.
Thermal stability
The ability of an enzyme to retain its catalytic power at high temperatures.
Chemical reaction
A process in which bonds are broken and new bonds are formed, resulting in the transformation of one or more substances into different substances.
Rate of reaction
The amount of product formed per unit time.
Catalysed reaction
A reaction that is accelerated by the presence of an enzyme, resulting in a much higher reaction rate compared to an uncatalysed reaction.
Metabolic pathway
A series of enzyme-catalysed reactions that convert a starting molecule (substrate) into a final product.
Substrate
The molecule that binds to the active site of an enzyme and undergoes a chemical or metabolic conversion.
Product
The molecule that is formed as a result of a chemical or metabolic conversion catalysed by an enzyme.1. Transition state:An intermediate structural state between the stable substrate and the product in a chemical reaction.
Activation energy
The difference in average energy content between the substrate and the transition state in a chemical reaction.
Enzyme
A protein that acts as a catalyst in biological reactions.
Substrate
The molecule that binds to the active site of an enzyme and undergoes a chemical reaction.
Enzyme-substrate complex
A short-lived complex formed when the substrate binds to the active site of an enzyme.
Enzyme-product complex
An intermediate complex formed during the catalytic cycle of an enzyme, before the products are released.
Catalytic cycle
The series of steps involved in the enzymatic reaction, including substrate binding, shape alteration, bond breaking, product formation, and enzyme regeneration.
Optimum temperature
The temperature at which an enzyme shows its highest activity.
Optimum pH
The pH at which an enzyme shows its highest activity.
Denaturation
The process in which proteins lose their structure and function due to high temperature or extreme pH.
Vmax
The maximum velocity of an enzymatic reaction, reached when all enzyme molecules are saturated with substrate.