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Define a population
A group of organisms of the same species living in an area at one time
Give two methods of estimating population size
Sampling, capture-mark-release-recapture
What is sampling used to investigate?
Abundance and distribution of populations
Give two types of sampling
Random and systematic
When is random sampling the best choice?
When a sampling area is reasonably uniform
How is random sampling done?
Lay out a grid over area to be studied, generate random number coordinates, place sample sites in coordinates
What assumption does a population estimate based on sampling make?
Individuals are distributed evenly across the site
What is used to study distribution of sessile organisms?
Quadrats
Give four types of data that can be recorded from a frame quadrat
Presence or absence of a species, species frequency, species abundance, percentage cover
What is used to study distribution of motile organisms?
Capture-mark-release-recapture
Describe capture-mark-release-recapture
The first large sample is taken and individuals are counted and marked in a way that won't affect their survival. The marked individuals are returned to their habitat and allowed to randomly mix with the rest of the population. After sufficient time, another large sample is captured, and the number of marked and unmarked individuals are counted. The proportion of marked to unmarked individuals is used to calculate an estimate of population size using the Lincoln index.
Lincoln index formula
M x N/R M = initial marked N = total in second sample R = marked in second sample
Give the four assumptions the Lincoln index makes
The marked individuals disperse and mix back in fully with the population. The marking doesn't affect survival rates. The marking doesn't rub off. The population stays the same size.
Define carrying capacity (K)
The maximum number of individuals of a species that an ecosystem can support
Give three abiotic factors affecting carrying capacity
Light availability, temperature, soil mineral availability
Give three biotic factors affecting carrying capacity
Competition for resources, predation, disease
Density-dependent factors act on a ? feedback effect
Negative
Give an example of a predator-prey interaction affecting population
Canada lynx and snowshoe hare
Give two types of population control factors
Top-down or bottom-up
What type of population control is limitation by predators?
Top-down
What type of population control is limitation by availability of resources?
Bottom-up
Give two ways interspecific competition strategies work
Increasing survival chances of species (eg camouflage) or decreasing survival chances of competing species (eg allelopathy)
What is allelopathy?
Chemical inhibition of another species' growth by secreting secondary metabolites that harm other organisms
Give an example of a plant species that carries out alleloapthy
Garlic mustard producing sinigrin reducing seed germination and root growth in other plant species
Explain how antibiotic secretion functions as allelopathy
Some microorganisms secrete antibiotics to kill bacteria, reducing interspecific competition
Give the three phases of the sigmoid growth curve
Exponential (logarithmic), transition, plateau
What happens in the exponential phase of the sigmoid growth curve?
No factors that limit population growth, so population increases exponentially as number of individuals and rate of growth increases
What happens in the transition phase of the sigmoid growth curve?
Limiting factors start to act, so rate of growth slows even though population is still increasing
What happens in the plateau phase of the sigmoid growth curve?
Limiting factors cause death rate to equal birth rate so population growth stops at carrying capacity, although still fluctuates
Give two organisms used to model the sigmoid growth curve
Yeast, duckweed
Explain how yeast is used to model the sigmoid growth curve
Yeast is grown in a broth culture, an the turbidity of the suspension is used to estimate the number of cells by measuring how much light can pass through at fixed time intervals with a turbidity meter or a colourimeter.
Explain how duckweed is used to model the Sigmoid growth curve
Place a small number of duckweed fronts into a petri dish containing distilled water and liquid fertiliser in a brightly lit location. Record the number of fronds present each week for six weeks.
Why is duckweed ideal for modelling population growth?
Reproduces quickly and asexually, and is easy to count as in clusters.
Give the two types of intraspecific interactions
Cooperation adn competition
Give three examples of intraspecific cooperation
Orcas hunt together by creating a wave to wash seals into the water, meerkats have roles within the group, ants work together to build nests and find food
Give three examples of intraspecific competition
Male red deer fight for mating, robins defend territorially, oak trees compete for light, water, and minerals
Define a community
Multiple populations of different species living and interacting in the same area
Give six interspecific interactions
Herbivory, predation, interspecific competition, mutualism, parasitism, pathogenicity
Give an example of parasitism
Fleas on dogs feeding on blood
Give an example of pathogenicity
Dutch elm disease is caused by a fungal pathogen that causes elm trees to lose their leaves and die
Give an example of mutualism
Zooxanthellae and coral polyps
Explain the symbiotic relationship between zooxanthellae algae and coral polyps
Polyps have tentacles which contain the zooxanthellae cells, providing shelter and protection for the algae, while the algae carry out photosynthesis and produce carbon compounds
Define an invasive/alien species
A species moving into an ecosystem where it was previously unknown
Why does the population of invasive species rapidly increase?
No natural population controls eg no natural predators or competitors
Give three ways an invasive species can affect an ecosystem
Competition with native species in a similar niche, decline in prey species, introduce new diseases which native species have no natural immunity to
Give an example of an invasive species
Grey squirrels from North America in the 1800s in the UK, largely outcompeting red squirrels in interspecific competition
Define endemic species?
Species found naturally in only one geographic area and no place else
Why are endemic species especially vulnerable to the effects of invasive species?
Local extinction is entire extinction
Why are enzymes necessary? Give two reasons
Most reactions occur slowly, and this isn't fast enough to sustain life, so enzymes act as biological catalysts. Also, the reactants would need to collide at the correct angle and speed, the chance of which is very low without enzymes.
Define metabolism
All of the chemical reactions that take place within cells and organisms
Define a metabolic pathway
A series of interlinked metabolic reactions
What are enzymes?
Globular proteins, specific to a substrate that act as biological catalysts to increase the rate of reaction
Which two things are complementary in an enzyme-substrate complex?
Chemical properties and shape
What do anabolic reactions do?
Build large molecules from smaller ones
Give three examples of anabolic reactions
Photosynthesis, protein synthesis, glycogen formation
What type of reactions do anabolic reactions often include?
Condensation reactions
Are anabolic reactions endergonic or exergonic?
Endergonic (require input of energy to take place), resulting in energy-storing products
What do catabolic reactions do?
Break down large molecules into smaller ones
Give two reasons why catabolic reactions take place
To release energy for cellular processes and for the excretion of waste
Give three examples of catabolic reactions
Respiration, deamination of proteins to release urea, breakdown of macromolecules into monomers during digestion
What reactions do catabolic reactions often include?
Hydrolysis reactions
Are catabolic reactions endergonic or exergonic?
Exergonic (releasing free energy for cellular processes or as excess heat)
What is the modified model of enzyme activity known as?
Induced-fit hypothesis
Explain the induced-fit hypothesis
The enzyme and its active site can change shape slightly as the substrate molecule enters the enzyme, known as conformational changes, ensuring an ideal binding arrangement to maximise the ability of the enzyme to catalyse the reaction.
Give three ways an enzyme can be immobilised
Attachment to an inert substance (eg glass), entrapment within a matrix (eg alginate gel), entrapment within a partially permeable membrane
Give five benefits of immobilising enzymes
No enzyme in the product (uncontaminated) so no further processing to remove it is needed. The enzyme can be reused without later separation. Greater tolerance of temperature and pH changes as they are more stable. Substrates can be exposed to higher enzyme concentrations than in solution. Conditions can be controlled carefully.
Explain how denaturation of enzymes happens
When exposed to high temperatures or extremes of pH, the bonds holding the molecule in its shape start to break, causing the shape of the protein to change, changing the shape of the active site, preventing the substrate from bonding.
Give an overview of photosynthesis
Carbon dioxide is converted to glucose (using hydrogen released by splitting a water molecule) releasing oxygen as a waste product
Give three types of organisms which carry out photosynthesis
Plants, algae, cyanobacteria
Explain how light energy leads to photosynthesis
Within the chlorophyll, light energy results in the excitation of electrons, triggering transfer of electrons, leading to a series of reactions which makeup photosynthesis in which light energy is transformed to chemical energy
Which two regions of the light spectrum do chlorophylls absorb wavelengths from?
Blue-violet and red
Which region of the light spectrum do carotenoids absorb wavelengths from?
Blue-violet
Which end of the visible light spectrum has the longest wavelength?
Red
Give two carbon dioxide enrichment experiments
Enclosed greenhouse experiments and free air carbon dioxide enrichment experiments
Why are carbon dioxide enrichment experiments important?
Establish the effect of carbon dioxide on plant growth and photosynthesis to develop a clearer idea of potential future risks
How do FACE experiments work?
Carbon dioxide is pumped into a natural ecosystem to increase localised carbon dioxide concentrations.
Give one benefit and one drawback of FACE compared to greenhouse experiments
Larger plants and trees can be studied, but other variables cannot be controlled (can be monitored though)
Define a habitat
The place where an organism lives
Give two ways in which a habitat can be described
Geographical location and type of ecosystem
Give eight abiotic factors
Light intensity, temperature, water turbidity, humidity, soil/water pH, soil/water salinity, soil composition, oxygen/carbon dioxide concentration
Give two species as examples for adaptions to abiotic factors
Marram grass and mangrove trees
What abiotic factors are in the habitat where is marram grass found?
Low water availability, high salinity, low nutrient levels
Give four adaptations of marram grass
Adaptations to reduce steep concentration gradient by raising humidity in air surrounding leaf to reduce water loss by evaporation: Leaves are rolled up to reduce exposure of surfaces to the wind, trapping water vapour in the rolled leaf. The stomata are in sunken pits to trap water vapour. The inner surface of the leaf is covered in tiny hairs which trap water vapour. Also, marram grass leaves have a thick waxy cuticle on their outer surface to reduce evaporation.
What type of plant is marram grass?
A xerophyte
Give the abiotic factors found in mangrove swamps
High salinity, low fresh water availability, low oxygen availability (bc submerged in water)
Give the adaptations of mangrove trees
Prop roots, providing stability, pneumatophores growing vertically out of water-logged soil specialised for gas exchange, cells don't allow entry of salt and prevent outward movement of water, or excrete excess salt through salt glands
Define a limiting factor
Any biotic or abiotic factor that restricts the growth of organisms
Define distribution
Where a species is found within an ecosystem
Give a method of investigating limiting factors and species distribution
Transect sampling
What is a transect used for?
A transect is used to measure how the abundance of a species changes along an environmental gradient
What are transects?
Lines laid out across a site
Give four types of transects
Continuous line transect (every species touching the tape measure is recorded), interrupted line transect (species touching the line at regular intervals are recorded), continuous belt transect (quadrats are placed end-to-end along the line), interrupted belt transect (quadrats are placed at regular intervals)
How is rainfall measured?
Rain gauge
How is humidity measured?
Hydrometer
How is dissolved oxygen measured?
Electronic oxygen sensor
How is water turbidity measured?
Turbidity meter
How is light intensity measured?
Electronic light meter
How is landscape relief measured?
Contour lines on a map or a GPS
How is site aspect (direction site faces) measured?
Compass
How is slope incline measured?
Clinometer