Proteins of DNA Replication

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7 Terms

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helicase

unwinds the parental double helix at replication fork

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single-stand binding protein

binds to and stabilizes single-stranded DNA until it’s used as a template

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topoisomerase

relieves overtwisting strain ahead of the replication forks by breaking, swiveling, and rejoining DNA strands

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primase

synthesizes RNA primer at 5’ end of leading strand & at 5’ end of Ozaki fragment of lagging strand

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DNA pol III

uses parental DNA as template to synthesize new DNA stands by adding nucleotides to an RNA primer or pre-existing DNA strand

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DNA pol I

removes RNA nucleotides of primer from 5’ end & replaces them with DNA nucelotides

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DNA ligase

joins Okazaki fragments of lagging strand; on the leading strand it joins 3’ end of DNA that replaces the primer to rest of the leading DNA strand