Bio lab 4 fingerprinting

Restriction Digestion

Restriction digestion is a process in which DNA is cut at specific sites
by restriction endonucleases, dictated by the surrounding DNA
sequence.

DNA Fingerprinting

DNA fingerprinting is a technique used to determine the probable
identity of a person based on the nucleotide sequences of certain
regions of human DNA that are unique to individuals.
• DNA fingerprinting is used in:
criminal investigations,
other forensic purposes
paternity testing

These molecular scissors or “cutting” enzymes are

restriction endonucleases.

recognition sites.

In 1968, Dr. Werner Arber at the University of Basel, Switzerland, and Dr. Hamilton Smith at the Johns Hopkins University, Baltimore, discovered a group of enzymes in bacteria that break (hydrolyze) the sugar-phosphate backbone at specific sites called

—HOW CAN WE DETECT DIFFERENCES IN BASE SEQUENCES?

At first sight, your task might seem rather difficult. You need to determine if the linear base pair sequence in the DNA samples is identical or not. An understanding of some relatively recent developments in recombinant DNA technology might help you to develop a plan