HEMATOLOGY QUICK NOTES (ASCP)

WBC

- 4.5-11

RBC

- Male: 4.6-6

- Female: 4-5.4

HGB

- Male: 14-18

- Female: 12-15

HCT

- Male: 40-54%

- Female: 35-49%

MCV

- 80-100

MCH

- 27-31

MCHC

- 32-26%

Platelet

- 150-450

RDW

- 11.5-14.5%

Retic

- 0.5-1.5%

Hemoglobin A

- 2 alpha or beta chains

- >95%

Hemoglobin A2

- 2 alpha or delta chains

- 1.5-3.7%

Hemoglobin F

- 2 alpha or gamma chains

- <2%

Hemoglobin S

- Valine substituted for glutamic acid in 6th position of β chain

- 0%

Hemoglobin C

- Lysine substituted for glutamic acid in 6th position of β chain

- 0%

Sickle cell (SS)

- Micro: Sickle cells, target, nRBCs, poik, aniso

- HGB: >80% S, 1-20% F, normal A2, no A

Sickle cell trait (AS)

- Micro: Occasional target, no sickle cells

- HGB: 50-65% A, 35-45% S, normal F and A2

Hemoglobin C Disease (CC)

- Micro: Many targets, hgb C crystals, folded cells

- HGB: >90% C, <7% F, no A

Hemoglobin C trait (AC)

- Micro: Many targets

- HGB: 60-70% A, 30-40% C

SC Disease (SC)

- Micro: Targets, SC crystals, boat shaped cells

- HGB: > S than C, normal F, no A

Hereditary Spherocytosis

- Micro: Polychromasia, spherocytes

- Increased retics and MCHC

Iron Deficiency anemia (IDA)

- Micro: Ansio, poik, hypochromic, microcytosis

- Increased: RDW and TIBC

- Decreased: RBC, Serum iron, and ferritin

Cellulose acetate

- -CSFA+

Citrate agar

- +CSAF-