Enzyme Kinetics Lab: Percent Inhibition Calculation

Flashcards covering key concepts from enzyme kinetics and percent inhibition calculations.

Percent Inhibition

A measure of how much the inhibitor reduces the product formation compared to the control with no inhibitor.

Normalized OD

The optical density values adjusted by subtracting the OD of the no-substrate control.

Standard Curve

A graph used to convert optical density values into product concentration.

Inhibitor Concentration

The amount of inhibitor present in a reaction, measured in mM (millimoles per liter).

Product Formation

The amount of product generated in a reaction over time.

No-Substrate Control

A control reaction without substrate used to measure the baseline optical density.

Calculation Method for % Inhibition

% Inhibition = (1 - (Product Formation Inhibitor / Product Formation No Inhibitor)) × 100

Negative Inhibition Values

Values that are reported as zero percent inhibition if calculated inhibition is negative.

Graph of % Inhibition vs Inhibitor Concentration

A visual representation showing the relationship between the percent inhibition and the concentration of the inhibitor.

Bar Graph

A type of graph suitable for comparing the percent inhibition across different inhibitor concentrations.