BCE 9&10_Weird and wonderful bioreactors, modes of operation, and novel synthetic platforms.pdf

Stirred Tank bioreactor

Stirred Tank bioreactor

most commonly used types

Fundamentals of bioreactor design

O2 mixign and transfer, shear stress control and meet growth requirement

non-standard production requirement

small volume production, unsusal substrate and cleaning requirements

Airlift reactor

multi-gas input for augment mixing

ADVANTAGE or airlift reactors

DISADVANTAGE of airlift reactors

Wave bioreactors

DISADVANTAGE wave reactor

ADVANTAGE wave reactor

Bioreactors for Adherent Cells

Plate Stack/ Membrane Bioreactors, Stirred Tank with Microcarrier

Adherent cells (ADHERE)

Cell that sticks, grow once adhere

Plate Stack

Membrane bioreactors

ADVANTAGE Plate Stack/Membrane Bioreactors

DISADVANTAGE Plate Stack/Membrane Bioreactors

Microcarrier-based bioreactors

Attach cells to particle in low density, provide the surface for growth

Microcarriers

round polymeric objects for cell attachment

microcarrier types

porous and non-porous

specifying microcarriers

change the protein covered on the surface

microcarrier possible vessles

stirred tank, airlift, fluidised bed

ADVANTAGE of microcarrier system

DISADVANTAGE of microcarrier system

Photobioreactors

grow algae/ suspension culture, grow phototrophically

Photobioreactors ADVANTAGE

Photobioreactors DISADVANTAGE

Key challenge is to ensure good light penetration with high density of culture

Tray Fermenter

for Solid State Fermentations

Tray fermenter mechanism

trays containing organisms and solid substrate,

Solid growth substrate with minimal water consumption

Organism for Tray fermenters

Fungal cultures

Tray fermenters advantageous

Tray fermenters DISadvantageous

Micro/Nano Bioreactors

Small volume, name relative to the bioreactor vessel size itself

Micro/Nano Bioreactors ADVANTAGE

Reduces cost, saves time
parallel investigations

Micro/Nano Bioreactors DISADVANTAGE

non-linear scale-up challenges: not always accurately mimic larger scale

Disposable Single-Use Bioreactors

Exactly what it said

Single use bioreactors ADVANTAGE

Single use bioreactors DISADVANTAGE