[PHARMACOGNOSY] Identification Test for Glycosides

Liebermann's - Burchard Test

Unsaturated steroidal or cardiac glycoside

Result: Green color

Borntrager's Test

Anthraquinone glycoside

Result: Reddish coloration with alkali

Flavonoidal Glycoside

Result: Characteristic color with NH4OH, AlCl3, FeCl3

Bate Smith and Metcalf Test Method

Leucoanthocyanins

Result: Strong-red or violet

Sodium Picrate Paper Test

Cyanogenic glycosides

Result: From yellow to red color

Treatment with AgNo3

Sulfur Containing glycosides

Result: Black precipitate of silver sulfate

Wilstatter Test - aka Cyanidin Test

orange to red, crimson and magenta and occasionally to green or blue indicates the presence of benzopyrone nucleus.

Froth Test

Agar Cup Method Hemolytic Test

Saponin Glycosides

Keller Killiani Test

Test for deoxysugars

Glacial acetic acid containing + FeCl 3+ H2SO 4→ brown ring free from red (acetic acid = blue).