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What are the steps of the scientific method (in order)?
Observation → Curiosity → Question → Hypothesis → Experiment → Data/Observations → Results → Analysis → Conclusion → Theory
What is a hypothesis?
A testable, falsifiable explanation for an observation
What is a theory?
A well-supported explanation backed by repeated experiments and evidence
qualitative data
descriptive
quantitative data
numerical with units
What is a positive control?
Shows expected results to confirm experiment works
What is a negative control?
Shows no effect to ensure results aren’t due to outside factors
What are the characteristics of life?
Made of cells, metabolism, homeostasis, grow & develop, reproduce, respond to stimuli, evolve
Are viruses living?
No—lack cells and metabolism, require host
What defines a prokaryotic cell?
No nucleus, small, simple, DNA in cytoplasm (bacteria)
What defines a eukaryotic cell?
Nucleus, membrane-bound organelles, larger, complex
What structures do all cells have?
DNA, ribosomes, cytoplasm, cell membrane
What is the cell membrane made of?
Phospholipid bilayer with embedded proteins
Why is the membrane selectively permeable?
Controls what enters and leaves the cell
What is diffusion?
Movement from high → low concentration (no energy)
What is facilitated diffusion?
Diffusion using transport proteins (no energy)
What is osmosis?
Diffusion of water across a semipermeable membrane
Define isotonic, hypertonic, hypotonic
Isotonic: equal solute → no net change
Hypertonic: more solute outside → cell shrinks
Hypotonic: less solute outside → cell swells/lyses
What is active transport?
Movement from low → high concentration using ATP
Endocytosis vs exocytosis?
Endocytosis = bring in; Exocytosis = release out
What do enzymes do?
Speed up chemical reactions
How do enzymes work?
Lower activation energy
What affects enzyme activity?
Temperature, pH, substrate concentration
What happens if enzymes denature?
Shape changes → enzyme stops working
Difference between inhibitors & activators?
Inhibitors slow/stop enzymes; activators increase efficiency
What elements make up most macromolecules?
CNSHOP (carbon, nitriogen, sulfur, hydrogen, oxygen, phospurase)
Monomer of carbohydrates?
Monosaccharides (glucose)
Function of carbohydrates?
Quick energy, structure, cell communication. Examples: Starch, glycogen, cellulose
Lipid building blocks?
Glycerol + fatty acids
Functions of lipids?
Long-term energy, membranes, hormones, insulation. Examples: Fats, oils, steroids, phospholipids
Monomer of proteins?
Amino acids (20 types)
Functions of proteins?
Enzymes, structure, transport, movement, defense. Examples: Enzymes, hemoglobin, collagen
Monomer of nucleic acids?
Nucleotides (A, T, C, G, U)
Function?
Store & transmit genetic information. Examples: DNA, RNA
Function of the nucleus?
Stores DNA; controls cell activities
Function of ribosomes?
Protein synthesis
RER vs SER?
RER: proteins; SER: lipids & detox
Golgi apparatus function?
Modifies, sorts, packages proteins
Mitochondria function?
Cellular respiration; ATP production
Lysosome function?
Digestion & recycling
Vacuole function?
Storage; maintains turgor pressure (plants)
Chloroplast function?
Photosynthesis (plants only)
Why do cells divide instead of growing large?
High surface area-to-volume ratio = efficient transport
What happens if cells get too large?
Nutrient & waste exchange slows
What is ATP?
Cell’s main energy molecule
What is homeostasis?
Maintaining stable internal conditions
What is the fluid mosaic model?
Membrane is flexible with moving proteins
What are cytoskeleton functions?
Shape, movement, transport within cell