MEDICAL INTERVENTIONS

What is the definition of medical intervention?

any measure whose purpose is to improve health or alter the course of disease

What is NOT an example of a medical intervention?

allergy

Sue was diagnosed with

neisseria meningitus

What would be least likely to contract meningitis?

hiking in the backwoods

What are unique symptoms of meningitis?

sensitivity to light

stiff neck

high fever

What part of the body does meningitis affect?

membranes of the brain and spinal cord

What are the correct steps to use DNA in pathogen identification?

prepare sample from patient and isolate DNA

make copies of the desired piece of DNA

sequence the DNA

analyze the sequence of DNA and identify the pathogens

What is the purpose of polymerase chain reaction (PCR)?

make many copies of a piece of DNA

Define pathogen

a foreign substance that causes an immune response

What is the definition of bioinformatics?

the collection, classification, storage and analysis of biological information using computers

During DNA replication, 1 original strand of DNA is copies into 2 identical strands of DNA. Each newly sythesized strand is made up of 1 original strand of DNA and 1 newly syntheized strand of DNA. This type of DNA replication is said to be

Semi-Conservative

During DNA replication primers are needed to

start the replication on each strand of DNA

The immune system is responsible for producing antiodies. What specific cell type makes antibodies

B-cells

What is the function of antibodies?

mark foreign substances for the immune system to attack

The ELISA test that we performed in class was testing the patient's sample for the presences of

antibodies

What does ELISA stand for?

Enzyme linked immunosorbent assay

In the ELISA test, what does the secondary antibody have?

an enzyme that catalyzes a reaction that causes a color change

What is a real world application of the ELISA test?

home pregnancy test

What does the ELISA test determine?

if you have the antibody and how much of it you have

What can you determine about the patient if the ELISA test is a false positive?

nothing, the test had inconclusive results

Which of the following is true about antibodies?

they are specific to one antigen

what can be a high concentration of antibodies in a patient's system?

1/100 dilution

If quick results are needed, why is the ELISA for antigen used insted of the ELISA for antibodies?

it takes a while for enough antibodies to build up to be detected

What is BLAST used for?

determine the type of organisms using the sequence of DNA

What is the correct order for the ELISA test that we did in class

add patient sample to tubes

add antigen to tubes

add primary antibody

add enzyme