Micro bio lab exam 1-LAB SAFETY

You may eat a snack in lab as long as there are no bacterial cultures on your bench and you have thoroughly washed your hands.

False

Which of the following would be most appropriate for disposal of cultures on agar plates?

Biohazard bag or bin

The best source of information regarding the hazards posed by any chemical and specific handling instructions is the

Material Safety Data Sheet (MSDS)

Which is the most appropriate disposal for a swab used to culture your throat during lab?

biohazard bag

Which of the following represent important safety procedures for microbiology students?

(Check all that apply)

-always wear open-toed shoes so organisms do not become trapped in shoes

-wash your hands before and after lab procedures

-disinfect your lab bench at the beginning and end of the lab period

-only drink a closed container of water while conducting lab

-take extra care around the flame

-wash your hands before and after lab procedures

-disinfect your lab bench at the beginning and end of the lab period

-take extra care around the flame

Thoroughly washing hands will sterilize them.

False

How long should the hands be lathered with soap and water?

20 seconds

Which of these steps will prevent the immediate recontamination of the hands after washing?

using a paper towel to turn off the faucet handle

when should soap be added to the hands?

after the hands are wet

if gloves are worn in lab, hand washing is unnecessary

false

Which of the following items may be on the laboratory bench top while doing lab work? (Check all that apply)

-cultures

-equipment items specifically needed to complete the lab activity

-backpack, purse

-pencil or pen

-cell phone

-cultures

-equipment items specifically needed to complete the lab activity

-pencil or pen

Using a cell phone in lab is permissible since it can be used for timing experiments.

FALSE

Jackie went to the sink at the end of lab to wash her hands. After removing her watch and rings, she turned on the water, wet her hands, added soap and thoroughly rubbed and lathered her hands for 30 seconds, cleaning the backs of her hands, her fingernails, and between her fingers. She then turned off the water, and reached for the paper towels to dry her hands. Identify the correct statement below.

Jackie should have used paper towels to turn off the faucet.

Beverages are allowed in the laboratory as long as the containers are covered.

False

Place the following steps of the hand washing procedure in the correct order.

1. Dry hands thoroughly and use a towel to turn off the faucet

2. Lather for at least 20 seconds by rubbing hands together paying attention to the back of hands, nails, and between fingers

3. rinse thoroughly in running water

4. Thoroughly wet hands in running water

5. apply soap

4, 5, 2, 3, 1

In which circumstance is food and/or beverage allowed in the laboratory?

Never

Paper towels should be discarded in a biohazard container after washing hands.

False

Jacob had just finished his lab work and went to the sink to wash his hands. He removed his watch, and added liquid soap to his hands. He then turned on the water and began to rub his hands together, lathering for 20 seconds and cleaning fronts and backs of hands, fingernails, and between his fingers. He then rinsed his hands in running water, dried his hands with paper towels, and used an additional paper towel to turn off the faucet. Which statement is correct?

Jacob should have wet his hands in running water before applying the soap

A disinfectant is used on your work surface:

-before the beginning of laboratory procedures

-after all work is complete

-after any spill of live microorganisms

-all of the above are correct

all of the above