BCHEM Final Exam

pK values for Tyrosine?

2.15

9.52

pk3 = 10.64

pk values for Cysteine?

2.15

9.52

8.37

pk values for Lysine?

2.15

9.52

10.54

pk values for Arginine?

2.15

9.52

12.48

pk values for Histidine?

2.15

9.52

6.04

pk values for aspartic acid?

2.15

9.52

3.90

pk values for glutamic acid?

2.15

9.52

4.07

Dansyl Chloride

Cuts N terminus only if K

Edman degradation

Cuts N terminus always

Cyanogen Bromide

Cuts C term M

O-Iodosobenzoate

Cuts C term side of W

Hydroxylamine

Cuts N-G bonds only

2-Nitro-5-thiocyanobenzoate

Cuts N term side of C

Trypsin

Cuts C term side of R, K, as long as no C term P

Chymotrypsin

Cuts C term side of F, W, Y, as long as no C term P

Elastase

Cuts C term side of V, G, A, S, as long as no C term P

Thermolusin

Cuts N term side of I, F, W, Y, V, M, as long as no N term P

Pepsin

Cuts on N term side of L, F, Y, W, as long as no N term P

Endopeptidase V8

Cuts C term side of E always

Acid Hydrolysis

Breaks down S, T, W, Y. Changes N to Q and D to E

Base Hydrolysis

Breaks downs C, S, T, R