Tests in Bacteriology Chap 14-18

Catalase Test Reagent

3% H2O2

Tube Coagulase reagent

Rabbit plasma

Hippurate hydrolysis reagent

Ninhydrin

LAP reagent

Leuvine-b-napthylamide

PYR reagent

Pyrrolidonyl-a-naphthylamide

Bacitracin Susceptibility Test purpose

Presumptive test for Streptococcus pyogenes

CAMP test purpose

presumptive test for S. agalactiae (GBS)

CAMP Factor

Hemolysis Enhancer

Hippurate Hydrolysis test purpose

differentiates GBS from other beta-hemolytic streptococci

PYR hydrolysis test purpose

Presumptive ID of GAS and nonhemolytic enterococci

LAP test purpose

Differentiate Aerococcus and Leuconostoc spp. from other GPC (NEG=Aero and Leuc)

Bile-esculin agar test purpose

Differentiates Enterococcus and GDS from other GPC

Optochin/P disk test purpose

Presumptive for S. pneumoniae

Neufeld Test/Quellung Rxn test purpose

Identifying encapsulated Strep spp.

Bile solubility test purpose

Presumptive ID of S. pneumoniae

6.5% NaCl Tolerance test purpose

Differentiates GDS from Enterococci

Novobiocin Susceptibility test purpose

Identification of Staph. saprophyticus

Mannitol Salt Agar

Differentiates Staph. aureus and Staph. epidermidis

Elek Test purpose

Identify the diphtheria toxin producing C. diphtheriae

Urease test purpose

detection of C. diphtheriae

Cytochromase oxidase test reagent

N, N, N’, N’ tetramethyl-p-phenylenediamine dihydrochlorine

Cystine Trypticase agar (CTA) test purpose

tests ability of bacteria to ferment glucose, maltose, lactose, and sucrose

Cytochromase oxidase test purpose

Testing for enzyme that oxidizes cytochrome C (which can transfer electrons to oxygen during aerobic respiration)