Colony morphology and Laboratory Analysis

Colony morphology Mold (4)

1. Room temperature

2. Quick: 3-5 days

3. Slow: 2-5 weeks

4. Hold for 4 weeks before calling it - if don’t grow

Colony morphology Yeast (2)

1. Physiological temperature (37C)

2. 48-72 hours (Faster than mold)

Media types (3)

1. Non-selective

2. Selective

3. Specialized yeast media

Non-selective media (3)

1. Sabouraud

2. Potato dextrose

3. Potato flake

Sabouraud

pH: 5.6 - inhibit most bacteria

Potato flake (3)

1. Wussy, non-nutritive medium

2. Asexual reproduction goes cray cray

   1. See unique patterns

Selective media

Mycobiotic agar

Mycobiotic agar (2)

1. Sabouraud + cycloheximide + chloramphenicol

2. Nasty bacteria grow slow and grow on this

Specialized yeast media (2)

1. Corn meal agar

2. Chromogenic

Corn meal agar (3)

1. Not used to grow from patient specimine

2. Help identify yeast genus species

3. Wimpy = odd asexual reproduction

Chromogenic

Identifies 4 organisms (The TEES)

Culture manipulation (What materials do we use and why) (4)

• Glass is hard to break

  • Breaking = Spreading = get into the lab and people

• Coccidioides

  • Dangerous: has killed Milwaukee lab scientists

How to identify with culture manipulation (4)

1. What colony looks like front and back

2. What the cells look at (microscopic morphology)

3. Growth rate

4. Where does it grow/ growth distribution