Lab 3 Part 1 Microscope Investigations

Magnification

the number of times larger an image appears compared to its real size.

Total Magnification

is the objective x ocular (in our case, the ocular is 10X)

Resolution

the effectiveness by which two points can be perceived as distinct

Refraction

When light moves from one substance (air) to another (water), it slows down and bends

Index of Refraction

a physical property defined by the speed of light in a vacuum divided by the speed of light in the medium

Field of View (f.o.v)

the area visible when looking through a microscope (measure based on the diameter)

f.o.v1mag1=f.o.v2mag2

With the diameter for a f.o.v for one magnification, You can estimate the f.o.v at another magnification

Unknown Diameter of Field of View Equation

(mag. of known lens/mag. of "unknown" lens) x known diameter of f.o.v.

One meter in millimeters

1,000 millimeters (mm)

One millimeter in micrometers

1,000 (µm)

One meter in micrometers

1,000,000 (µm)

If you are converting mm to µm

you must end up with MORE µm because they are smaller

If you are converting µm to mm

you must end up with FEWER mm because they are larger

Estimating Cell Size

position the slide so that multiple cells are visible near the diameter of field of view; estimate the number of cells that fit on the diameter

Cell Size Equation

diameter of f.o.v.(µm)/number of cells = approximate # of cells