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First step
find the correct BP order you want
Second
take the unkown single sided DNA you want to find
Then add
primers'
DNA polymerase (TAQ)
free nucleotides
What is the first type of free nucleotides
Normal- dNTP they can’t be seen by naked eye
what is the second type of free nucleotide
flurescent (can be seen) so you can tell what the coding is, codes for stop as well