Retest BB, Hemo, Coagule, Urine

define alloantibodies

antibody made from foreign response

define autoantibodies

antibody made from response to self antigen

allergy ad parasitic infections are caused by what Ig

IgE

what secretes CD4

T helper differentiates B cell

what secretes CD8

T suppressor and cytotoxic

what is B lymphs responsible for

humoral immune response

what cells are seen in bacteria and chemotactic

neutrophils

hat cells are seen in parasites and IgE

eosinophils

what cells are seen in histamine, heparin, and anaphylactic reactions

basophils

what is innate immunity

rapid response with physical defenses and cellular defenses like NK cells seen from birth

what is adaptive/acquired immunity

slower response that recognizes the foreign material

what is a humoral response

antibodies against infection creating memory

what is cell mediated response

cytotoxic cells against infection

what is active artificial immunity

vaccine with antigen

what is active natural immunity

disease

what is passive artificial immunity

AB injections

what is passive natural immunity

mothers milk or placental transfer

anti platelet is what disease

purpura

anti IF is what disease

pernicious anemia

what parasite is used in ANA testing

crithidia lucilae

is ANA is negative, what is ruled out

SLE

targets of HIV

CD4 t lymphs

what is used to test HIV

ELISA or HIV-1/2 antigens

polyspecific AHG has what antibodies

IgG, C3d and anti C3b, C4b, C4d

when do you use mono AHG

determine proteins in a positive DAT and in IAT when serum has both complement and non complement

how do you confirm a neg DAT

add Coombs control

what are the uses of DAT

HDN, AIHA, drug hemolytic anemias, transfusion reactions

what causes HDN

maternal IgG cross placenta and bind to fetal RBC

what is AIHA

autoantibodies target own RBC antigens leading to destruction

what does a DAT do for transfusion reactions

a pos DAT means recipients antibodies are reacting to the donors RBCs

what is the IAT used for

in vitro test. It checks for unbound antibodies that are floating in a person's blood plasma in a lab setting.

what is the DAT used for

in vivo test. It checks for antibodies that have already attached to a person's red blood cells inside their body.

can plasma be used to detect complement in IAT

no since plasma has anticoagulants that bind calcium and inactivate complement

what are the temp requirements for anti globulin tests

37C incubation for IAT

RT for DAT

IgM antibodies are associated with what type of antigen panel and blood groups

cold antibodies

ABO, Lewis (Le), MN, P, H, Lua

(Mint Cold)

IgG antibodies are associated with what type of antibody panel and blood groups

warm/37C

Rh, Duffy (Fy), Kell (Kp), Kidd (Jk), S, Lub

(Gas gets Warm)

which antigens react to enzymes

Rh, P, I, Lewis Kidd

(Raw Pineapple, Lemon, Ice, Kiwi)

which antigens are destroyed by enzymes

Duffy, MNS

(Duff Man Destroys)

what does dosage look like

2 of same antigen/homozygous

how are blood types inherited

codominate

which blood group systems have dosage

Rh, MNS, Duffy, Kidd

(Duff Mann Kidd R)

who first discovered the D antibody

levine and stetson 1939

who named D antigen

landseiner and Weiner

what is the most important antigen after A and B

D

donated blood that test neg with anti D but pos for Weak D should be labeled as what

Rh POS

if you are weak D, what blood should you receive

Rh NEG to prevent sensitization

if a patient was pos for an antigen but is no longer, what blood should they receive

Neg (whatever they currently have)

what is the most common class of immunoglobulin

IgG

what is the most important antigens

ABO then Rh

how does maternal antibodies affect HDN

maternal anti-D antibodies coat the newborns D antigens

what sugar does H create

L fucosyltransferase

what sugar does A create

N-acetyl

what sugar does B create

D-galactose

when do antibodies start

after birth due to environment

how can anti-A1 be used for A subgroups

the antisera reacts with A1 but not A2

what is the preferred plant lectin for A1 and A2

Dolichos biflorus agglutinates A1 not A2

what is the primary ABO antibody

IgM does not cross placenta

which ABO cross palcenta

IgG which can cause HDN

what antigen antibody does group A have

A antigen but B antibody

what antigen antibody does group AB have

A and B antigen with no antibody

what antigen antibody does group O have

no antigen but both antibodies

where is anti-B made

group A individuals

where is anti A,B made and why is it dangerous

made in group O as IgG so it can cause HDN

how does leukemia affect ABO

decrease in ABH antigens

what is cell grouping

forward testing patient antigens with known antibodies

what is the ABO based on the cell grouping:

anti-A + but anti-B -

A antigen is present since it reacted to the antisera

group A

what is the ABO based on the cell grouping:

anti-A + but anti-B +

A and B antigen is present since it reacted to the antisera

group AB

what is the ABO based on the cell grouping:

anti-A - but anti-B -

No reaction so

group O

what is serum grouping

reverse testing patient antibodies using known antigens

what is the ABO based on the serum grouping:

A1 + but B -

A antibody is present since it reacted to the antigen

group B

what is the ABO based on the serum grouping:

A1 + but B +

A and B antibody is present since it reacted to the antigen

group O

what is the ABO based on the serum grouping:

A1 - but B -

No antibody so

group ABw

how do you fix a weak antibody

incubate at 4C for 30min due to cold agglutination

what is LeSe

Le(a-,b+) and Lea, Leb, ABH

what is Lele sese

Le(a+,b-) and only LeA

what is lele Se

Le(a-,b-) and ABH

what is le se

Le(a-,b-) and nothing

what is the normal blood pressure results

sys: 90-180

dia: 50-100

what is the normal pulse results

50-100

what is the normal temp results

<99.5

what is the normal weight results

>110

what are the 2 tests for donors

ABO/RH groups

antibodies

what antibodies are tested for donated blood

syphilis, HBsAg, anti-HBc, anti-HCV, HIV, T.cruzi (chagas), WNC, Zika, CMV

what is the purpose of just whole blood and packaged RBCs

replenish blood loss

what is the purpose of just deglycerolized and washed RBCs

avoid allergic transfusion

what is the purpose of just leukocyte poor RBCs

febrile transfusion

what is the purpose of just FFP

vit K deficiency

what is the purpose of just platelets

thrombocytopenia

used for Purpura

what is the purpose of just plasma

volume

what is the purpose of just cryo

fibrinogen and F8 and vWF

what is the purpose of just granulocytes

neutropenia/sepsis

WB and RBC are stored at

1-6 for 42 days

what blood component is stored at 20-24

platelets for 5 days is single, 4hours if pooled

what blood component is stored at -18C

FFP and cryo for 1 year

what blood component is stored at -65

glycerolized

CPDA1 and AS3 extends expiration by how much

35 days

CPD and CP2d extend expiration by how much

21 days

AS1//2 additives extend duration by how much

42 days

how long can thawed cryo last

a single is 6 hours

pooled is 4 hours

which blood component lasts 10 years

frozen RBC and glycerolized