Gel Electrophoresis

Central dogma

DNA → RNA →proteins

Electrophoresis

Relies on the principle, that when a molecule enters an electrical field, its mobility is influenced by the charge of the molecule

How do the molecules migrate and what do they form

The molecules of migrate to form distinct according to their molecular weight, shape, and charge

how does the molecular weight influence movement in gel electrophoresis ?

Large molecules tend to migrate slower, shorter distances, and smaller molecules

Agarose

A chain of sugar molecules

How does charge influence movement in gel electrophoresis?

Negatively charged die, will migrate to positive electrode (red) and positively charged die when migrate to negative electrode (black)