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What is the purpose of the nitrate reduction test?
to see if nitrate converts into nitrite
What is the media in the nitrate reduction test?
undefined liquid
What is the indicator in the nitrate reduction test?
no indicator
What are the reagents in the nitrate reduction test?
nitrate a, nitrate b, and nitrate c
What is the purpose of the oxidation-fermentation test?
to see if bacteria oxidizes and ferment carbohydrates
What is the media in the oxidase-fermentation test?
undefined semisolid media
What does the media in the oxidase-fermentation test contain?
high amount of glucose and low amount of peptones
What is the indicator in the oxidation-fermentation test?
bromothymol blue
What color does bromothymol blue reflect in a neutral pH?
green
What color does bromothymol blue reflect in an acidic pH?
yellow
What color does bromothymol blue reflect in a basic pH?
blue
What is the first test in the IMViC test?
indole test
What is the purpose of the indole test?
to see if bacteria produces indole and contains tryptophanase
What is the media of the indole test?
undefined TSBroth
What does the media in the indole test contain?
tryptophanase and soy
What is the indicator in the indole test?
no indicator
What is the reagent in the indole test?
kovac reagent
What is the second test in the IMViC test?
MRVP test
What is the purpose of the methyl red test?
to see if bacteria is a mixed-acid fermenter
What is the purpose of the Voges-Proskaeur test?
to see if bacteria ferments glucose through the butanediol pathway
What is the media in the MRVP test?
undefined liquid
What does the media in the MRVP test contain?
peptones, glucose, and a phosphate buffer
Why does MRVP have a phosphate buffer?
to ensure that only methylred can fail
What is the third test in the IMViC test?
citrate test
What is the purpose of the citrate test?
to see if bacteria can import and ferment citrate?
What does it mean if the citrate test is positive?
it means that citrate is the only carbon source
What is citrate permease?
an enzyme that brings citrate into the cell
What is citratelyase?
an enzyme that breaks down citrate
What is the media in the citrate test?
defined solid
What does the media in the citrate test contain?
ammonia, citrate, and bromothymol blue
What is the indicator in the citrate test?
bromothymol blue
Note 
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