IMViC, O-F Medium, & Nitrate Reduction Quiz

What is the purpose of the nitrate reduction test?

to see if nitrate converts into nitrite

What is the media in the nitrate reduction test?

undefined liquid

What is the indicator in the nitrate reduction test?

no indicator

What are the reagents in the nitrate reduction test?

nitrate a, nitrate b, and nitrate c

What is the purpose of the oxidation-fermentation test?

to see if bacteria oxidizes and ferment carbohydrates

What is the media in the oxidase-fermentation test?

undefined semisolid media

What does the media in the oxidase-fermentation test contain?

high amount of glucose and low amount of peptones

What is the indicator in the oxidation-fermentation test?

bromothymol blue

What color does bromothymol blue reflect in a neutral pH?

green

What color does bromothymol blue reflect in an acidic pH?

yellow

What color does bromothymol blue reflect in a basic pH?

blue

What is the first test in the IMViC test?

indole test

What is the purpose of the indole test?

to see if bacteria produces indole and contains tryptophanase

What is the media of the indole test?

undefined TSBroth

What does the media in the indole test contain?

tryptophanase and soy

What is the indicator in the indole test?

no indicator

What is the reagent in the indole test?

kovac reagent

What is the second test in the IMViC test?

MRVP test

What is the purpose of the methyl red test?

to see if bacteria is a mixed-acid fermenter

What is the purpose of the Voges-Proskaeur test?

to see if bacteria ferments glucose through the butanediol pathway

What is the media in the MRVP test?

undefined liquid

What does the media in the MRVP test contain?

peptones, glucose, and a phosphate buffer

Why does MRVP have a phosphate buffer?

to ensure that only methylred can fail

What is the third test in the IMViC test?

citrate test

What is the purpose of the citrate test?

to see if bacteria can import and ferment citrate?

What does it mean if the citrate test is positive?

it means that citrate is the only carbon source

What is citrate permease?

an enzyme that brings citrate into the cell

What is citratelyase?

an enzyme that breaks down citrate

What is the media in the citrate test?

defined solid

What does the media in the citrate test contain?

ammonia, citrate, and bromothymol blue

What is the indicator in the citrate test?

bromothymol blue