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Purpose of Forensic Science:
To use science to help resolve legal matters
Role of Forensic Investigator:
1. Collects and examines physical evidence
2. Reports to law enforcement
3. Possibly testifies in court
Tools of the Forensic Investigator:
1. Ability to observe, interpret, and report findings
2. Ability to identify the evidence, document it, and determine its significance
Role and Responsibilities of Forensic Scientists:
- Observation and application
- Communication
- Deductive Reasoning
Observation and application of Forensic scientists
1. Find evidence
2. Collect data
a. Qualitative data
b. Quantitative data
3. Analyze data
Communication of forensic scientists
1. Convince a jury that evidence is:
A. Scientific
B. Reliable
C. Valid
D. Sufficient
Deductive reasoning of forensic scientists
Using the scientific method and the claim-evidence-reasoning model
Scientific method:
1. State the problem or question
2. Propose a hypothesis
3. Conduct an experiment
4. Collect data
5. Analyze data
6. Draw a conclusion
Claim-Evidence-Reasoning Model:
1. Make observations
2. Determine what question you want to answer
3. Make a claim and an assertion
4. Provide evidence
5. Provide reasoning or justification
Observation
1. Gathering information through the senses
a. Sight, taste, hearing, smell, touch
2. Done largely without thinking
Perception
1. Interpreting sensory observations
Not always accurate
a. Does not always reflect reality
2. Understanding limitations
Helps improve observation skills
Observations made by witnesses:
1. Perception of witnesses can be faulty
2. Emotional states influence perceptions
3. Eyewitness accounts can be valuable evidence
4. Eyewitness accounts can be valuable evidence
5. Many factors affect accuracy
Eyewitness Accounts
1. Vary considerably from person to person
2. Require discrimination between fact and opinion
3. Used to sequence the events
4. The Innocence Project
5. Found that eyewitness identification contributed to up to 70 percent of wrongful convictions
Ways to improve observation skills:
1. Systematically examine the environment
2. Turn off filters; act like data-gathering robots
3. Avoid looking for patterns
4. Concentrate on gathering information
5. Leave interpretation until later
6. Write down and photograph as much as possible
Conducting Interviews
- Separate witnesses as soon as possible
- Help witnesses reconstruct what happened
- Provide minimal guidance
- Avoid interjecting biases
- Write down observations
- Ask questions to draw out details
Identifying a truth or a lie
- A true account is consistent each time it's told
- Lies are difficult to repeat exactly the same
- Methods to identify truths or lies
- Have witness repeat story several times
- Ask witness to recount story in reverse order
- Distract interviewee with questions
The Innocence Project
An organization staffed by lawyers and law students who reexamine cases and provide legal assistance to convicts when there is a probability that serious errors occurred in their prosecution.
Goals of a Crime-scene Investigation
- To recognize, document, photograph, and collect evidence at the crime scene
- Solving the crime depends on piecing together the evidence to form a picture of what happened at the crime scene
- Evidence must remain valid and reliable; in response to the 2009 National Academy of Sciences report calling for more scientific evidence testing, Scientific Working Groups (SWGs) were formed and later evolved into the Organization of Scientific Area Committees (OSACs) to standardize and improve protocols.
Professionals included on a crime scene
Police officers, district attorney, crime scene investigators (CSIs), medical examiners (coroners) detectives, and specialists
Police Officers
Typically the first responders who secure the area and ensure public and officer safety.
District Attorneys
Determine legal requirements, such as obtaining search warrants.
Crime Scene Investigators (CSIs)
Document the scene, take photographs, sketch layouts, and systematically collect evidence.
Medical Examiners (Coroners)
Examine the deceased to establish the cause and manner of death.
Detectives
Interview witnesses, pursue leads, and reconcile evidence findings with witness statements.
Specialists
Experts (such as entomologists, toxicologists, or botanists) brought in when specific evidence requires specialized analysis.
Locard's Principle of Exchange
- Whenever two people come into contact with each other or with an object, a physical transfer occurs
- The second part of this principle states that the intensity, duration, and nature of the entities in contact determine the extent of the transfer (e.g., more transfer occurs during a physical fight than from a brief brush-by).
Trace Evidence
Transferred materials (e.g., pet hair, fibers, fingerprints, blood drops, soil, paint chips, broken glass)
Direct Evidence
Firsthand observations such as eyewitness accounts or police dashboard video cameras
Examples of direct evidence
- A witness states that she saw a defendant pointing a gun at a victim during a robbery
- In court, this involves testimony by a witness about what that witness personally saw, heard, or did
- Confessions
Circumstantial Evidence
Evidence that implies a fact but does not prove it. No one other than the suspect and/or victim actually knows when this term is produced
Two types of circumstantial evidence
Physical and biological
Physical Evidence
Includes fingerprints, footprints, shoe prints, tire impressions, and tool marks
Biological Evidence
Includes DNA in tissue, bodily fluids, hair, plants, pollen, and natural fibers
Class Evidence
Narrows an identity to a group of persons or things
Example of class evidence
ABO blood type of a sample of blood from a crime scene tells us that one of many persons with that blood type may have been there
Individual Evidence
Includes a unique combination of characteristics that it could only belong to one person or thing, such as a fingerprint or DNA
The Seven S's of Crime Scene Investigation
1. Securing the scene
2. Separating the witnesses
3. Scanning the scene
4. Seeing the scene
5. Sketching the scene
6. Searching for evidence
7. Securing and collecting evidence
Securing the scene
- Is the responsibility of the first responding law-enforcement officer
- The safety of all individuals in the area is the first priority
- Preservation of evidence is the second priority
- The first officer on the scene keeps a security log of all those who visit the crime scene
Separating the witnesses
- Witnesses must not be allowed to talk to each other
- Crime-scene investigators will compare the witnesses' accounts of the events
- Witnesses are separated so that they do not work together to create a story (collusion)
- Interviewing the witnesses and asking specific questions
Questions to ask the witnesses when separating them
- When did the crime occur?
- Who notified law enforcement?
- Who is the victim?
- Can the perpetrator be identified?
- What did you see happen?
- Where were you when you observed the crime scene?
Scanning the scene
- Forensic CSIs determine where photos should be taken
- A determination may be made of a primary crime scene and a secondary crime scene, and priorities assigned regarding examination
Seeing the scene
- CSIs capture overall and close-up photos with and without a measuring ruler.
- Triangulation should be included in the photos
Triangulation
A technique used to record the location of evidence from two fixed reference points. Close-ups and photos of any evidence and remains from varying distances should be taken
Sketching the scene
- All objects should be measured from two immovable landmarks
- On the sketch, north should be labeled, and a distance scale should be provided
- Any other objects in the vicinity of the crime scene, such as doors, windows, and furniture, should be included in the sketch
Searching for evidence
- Types of search patterns are used depending on the size of the area to be searched and the number of investigators
- Single investigators might use a gridded, linear, or spiral pattern
- A group of investigators might use a linear, zone, or quadrant pattern
Securing and collecting evidence
- All evidence needs to be properly packaged, sealed, and labeled
- Specific procedures and techniques for evidence collection and storage must be followed
- Liquids and arson remains are stored in airtight, unbreakable containers
- Moist biological evidence is stored in breathable containers so that the evidence can dry out, reducing the chance of mold contamination
- After the evidence is allowed to air dry, it is packaged in a paper bindle, a folded paper used to contain trace evidence
- The bindle (also called a druggist's fold) can then be placed in a plastic or paper container
- This outer container is then sealed with tape and labeled with the signature of the collector written across the tape
- The size of the paper bindle depends on the size of the evidence
- If the evidence is small, the paper bindle can be constructed from a sheet of paper
- If the evidence is large, the bindle might be constructed from a large sheet of wrapping paper
- Evidenced to be logged
Steps that describe how to prepare a paper bindle for evidence collection
1. Choose the appropriate size sheet of clean paper for the bindle
2. Crease the paper as shown
3. Place evidence in the location shown by the X
4. Fold the left and right sides in
5. Fold in the top and bottom
6. Insert the top flap into the bottom flap; then tape the bundle closed
7. Place the bindle inside a plastic or paper evidence bag. Fold the bag closed
8. Place a seal over the folded edge of the evidence bag
9. Have the collector write their name over the folded edge
What should be in the evidence log
- Case number
- Item inventory number
- Description of the evidence
- Name of the suspect
- Name of victim
- Date and time of recovery
- Signature of person recovering
- Signature of person recovering the evidence
- Signature of any witnesses present during collection
Steps to map the outdoor crime scene
1. Establish the collection site
2. Locate a datum point
3. Mark evidence collection limits
4. Measure and mark evidence positions
Datum Point
Identify a permanent, immovable landmark (e.g., a tree or the corner of a building)
Subdatum Point
Using a compass, set a northern boundary corner at least 1 meter away from any evidence, mark it with a rebar or stake, and calculate the distance and angle from the datum
Perimeter Corners
Establish the remaining corners of the rectangular collection site using the subdatum point as a reference
Traingulation/Perpendicular Measurements
Map each piece of evidence by measuring its perpendicular distance to the North-South and East-West baseline perimeter lines
Analyzing the evidence
- A forensic lab processes all of the evidence that was photographed, documented, and collected at the crime scene
- The laboratory results are sent to the lead detective
- The detective examines the evidence and compares it to eyewitnesses' statements to check on the reliability of their accounts
- Evidence is evaluated to be scientific, sufficient, reliable, and valid
- The Department of Justice provides guidelines for expert witness testimony
- Evidence should be based on quantitative and statistical references and databases and should avoid inferences and subjective personal experiences
Crime Scene Reconstruction
Forming a hypothesis of the sequence of events from before the crime was committed through its commission
Commonly staged crime scenes
- Arson to cover other crimes
- Murder staged to look like suicide
- Burglary to collect insurance money
Determining if a crime scene is staged
- Initially treat all death investigations as homicides
- Are the type(s) of wounds on the victim consistent with the suspected weapon employed?
- Could the wounds be easily self-inflicted?
- Establish a victim profile
- Evaluate behavior before the event
- Corroborate statements
- Reconstruct the event
Advances in crime scene investigations
- Surveillance cameras and facial recognition programs have been very helpful in reconstructing accidents and identifying who was present and when they were present
- The use of ground-penetrating radar is used to locate bodies and hidden evidence
- Three-dimensional stationary camera systems like the OSCR 360 allow investigators to record crime scenes in real time and document the location of evidence for later viewing by juries
Jon Benet Rasmey Case
Key forensic evidence was lost because the crime scene was not properly secured
Mammalian species have two types of hair
1. Long and stiff hair
2. Fine, fuzzy, or wooly hair
Value of hair as evidence
Trace transfer and durability
Trace Transfer in hair
Easily adheres to surfaces (clothes, blankets, carpets) and transfers readily to victims, suspects, or crime scenes
Durability in hair
A tough outer keratin structure resists decomposition, remaining viable long after soft tissues decay
Classification of class evidence of hair
Hair without root/follicle provides characteristics (color, shape, medulla pattern) consistent with groups rather than a specific individual
Classification of individual evidence of hair
Hair with an attached root/follicle yields nuclear DNA unique to an individual
Late 1800s on hair
Hair recognized as trace evidence in criminal investigations; Alfred Swaine Taylor and Thomas Stevenson included a chapter on forensic hair analysis in The Principles and Practice of Medicale Jurisprudence (1883)
1910 on hair
Victor Balthazard and Marcelle Lambert published Le Poil de l'Homme et des Animaux, detailing microscopic studies of human and animal hair
1934 on hair
Dr Sydney Smith first utilized the comparison microscope for side-by-side comparisons of crime-scene hairs with known reference standards
Late 20th Century to Present on hair
Adoption of chemical profiling, Neutron Activation Analysis (NAA), mitochondrial DNA (mtDNA), and nuclear DNA typing
2015 Review on hair
The FBI, DOJ, Innocence Project, and NACDL reported that at least 90% of reviewed cases involving microscopic hair comparison contained examiner testimony errors, spurring stricter reliance on objective DNA and instrumental methods
Three Stages of the development of Hair
Anagen stage, catagen stage, and telogen stage
Anagen Stage
A period of active growth where the cells around the follicle are rapidly dividing and depositing materials within the hair, lasting approximately 1000 days, making up 80-90% of all human hair
Catagen Stage
Follows as the hair stops growing and the follicle recedes as the blood supply is reduced, making up about 2% of all hair growth and development
Telogen Stage
During this stage, the hair follicle is dormant or resting, and hairs are easily lost. At the end of this stage, the blood supply reconnects to the follicle, and another anagen phase begins. This stage makes up approximately 10-18% of all hairs
Hair Function
Thermoregulation (keeping warm or cool), environmental hazard protection, tactile sensation, and animal camouflage
Biological structures of hair
Follicles and hair shafts
Follicle
A club-shaped epidermal indentation containing the dermal papilla, blood-supplying capillaries, and actively dividing matrix cells with nuclear DNA. Associated with a sebaceous gland (containing oils) and erector muscles
Hair Shaft
Non-growing dead strand composed of keratin chains linked in helices
Shaft Cross-Section (Outer to Inner)
Includes cuticle, cortex, and medulla
Cuticle
Protective, scaly outer layer made of overlapping transparent cells
Cortex
Thickest middle portion in humans; contains pigment granules (melanin) determining hair color
Medulla
Central canal running down the hair axis (can be continuous, interrupted, fragmented, solid, or absent)
Macroscopic & Microscopic Human Hair
Varies in cross-sectional shape (circular, oval, flat), texture, color, and medullary pattern (continuous, interrupted/intermittent, fragmented/segmented, solid, or absent
Continuous medulla pattern of hair
One unbroken line

Interrupted (intermittent) medulla pattern of hair
Pigmented line broken at regular intervals

Fragmented or segmented medulla pattern of hair
Pigmented line unevenly spaced
Solid medulla pattern of hair
Pigmented areas filling both the medulla and the cortex
No medulla pattern of hair
No separate pigmentation in the medulla
6 Types of hair on the human body
Head, eyebrows, eyelashes, beard, moustache, underarm, body, and pubic
Head Hair
Uniform diameter, circular to elliptical cross section
Eyebrows and Eyelashes
Circular cross section with distinct tapered tips
Beard and Moustache hair
Coarse, thick, often triangular in cross section, sometimes featuring a double medulla
Underarm Hair
Straight, less buckled, long, fine, and sharp
Body Hair
Can be elliptical or triangular
Pubic Hair
Oval to triangular cross section; commonly displays marked diameter variations and buckling
Cosmetic Treatments
Bleaching oxidizes melanin throughout the cortex and distorts cuticle scales, displaying a sharp line of demarcation. Artificial dyes deposit pigment across the cuticle and cortex, creating an unnatural, painted appearance. Peroxide in bleach can also damage mtDNA
Growth Rate & PMI
Human head hair grows at roughly 1.3cm per month (0.44mm per day), allowing analysts to estimate the time elapsed since the last cosmetic coloring treatment
mtDNA
mitochondrial DNA
European Characteristics of Hair
- Appearance: Generally straight or wavy
- Pigment Granules: Small and evenly distributed
- Cross section: Oval or round of moderate diameter with minimal variation
- Other: Color may be blond, red, brown, or black
Asian Characteristics of Hair
- Appearance: Straight
- Pigment granules: Densely distributed
- Cross section: Round with large diameter
- Other: Shaft tends to be coarse and straight; thick cuticle; continuous medulla; color black