Lab 2: Microscope and Cell

type of microscope?

compound microscope

identify microscope parts

eyepiece (ocular lenses), interpupillar distance scale, objective lens, stage, iris diaphragm knob, slide holder, stage movement knobs

identify microscope parts

main light switch, light intensity control knob, coarse focus knob, fine focus knob, objective lenses, stage holder, stage, iris diaphragm knob, stage movement knob

function of eyepiece (ocular lenses)

10x magnifying power; allows user to view magnified image produced by microscope’s objective lens

function of interpupillar distance scale

adjusts the distance between the eyepiece to match the distance between the user's eyes

function of objective lens

forms magnified image of specimen on slide; has 4 magnifciation lenses

4 types of objective lenses

scanning objective (red), low power objective (yellow), high power objective (blue), and hi-dry objective (purple/dark blue)

magnification of (red) scanning objective lens

4x magnifying power and 40x total magnification

magnification of (yellow) low power objective lens

10x magnifying power and 100x total magnification

magnification of (blue) high power objective lens

40x magnifying power and 400x total magnification

magnification of (purple/dark blue) high-dry objective lens

60x magnifying power and 600x total magnification

function of main light switch

turns the microscope light source on and off (o is off and | is on)

function of light intensity control knob

adjusts intensity of the microscope’s light source from 1-6; “dimmer switch”

function of stage

where the slide is placed on the microscope

function of stage holder

holds the slide on the stage

function of stage movement knob

moves the slide on the stage up down, left right

function of iris diaphragm knob

regulates the amount of light that passes from the light source to the stage; adjust to match the numerical aperture (NA) of the objective lens

function of coarse focus knob

knob used to bring the object on the slide into appropriate focus; used ONLY with scanning power objective

function of fine focus knob

knob used to bring the object on the slide into appropriate more detailed focus; used with ALL objective lenses

what cells? what at pointer?

epithelial cells; pointer is at nucleus of a epithelial cell

what cells visible? what cell at pointer?

pointer: white blood cell (leukocyte)

erythrocytes (red blood cells) and leukocytes (white blood cells)

erythrocytes (red blood cells) don’t have nuclei

name cell structures

name functions of cell structures

name cell structure and its parts

nucleus, nuclear envelope, nucleolus, chromatin

name cell structures

name cell structures

name functions of cell structures

cell membrane (plasma membrane)

protects the cell and regulates what comes in and out of the cell

nucleus

control center of the cell; contains DNA

nuclear envelope

regulates what comes in and out of nucleus

nucleolus

produce the large and small ribosomal subunits

chromatin

genetic information

cytoplasm

everything inside the cell except the nucleus

cytosol

fluid that suspends the cellular elements

rough endoplasmic reticulum

produces membrane-bound proteins and membranes

smooth endoplasmic reticulum

calcium storage and lipid metabolism

ribosomes

site of translation (protein synthesis)

Golgi apparatus

packages and ships membrane-bound proteins

mitochondria

produces ATP

lysosomes

intracellular digestion

vesicles

intracellular transport

centrosome

involved in cell division

centrioles

involved in cell division, HINT: striped

peroxisomes

detoxification