Microbiology Lab - Intro to microscope stuf

What part of the microscope is this

Ocular lens

What does the ocular lens do

Re magnifies the image formed by the objective lens (10x)

What part of the microscope is this

Body tube

What does the Body tube do

Transmits the image from the objective lens to the ocular lens

What [part of the microscope is this

The arm

What part of the microscope is this

Objective lenses

What do the Objective lenses do

They are the primary lenses that magnify the specimen

What part of the microscope is this

Stage

What does the stage do

Holds the microscope slide in position

What part of the microscope is this

Condenser

What does the Condenser do

Focuses light through the specimen

What part of the microscope is this

Diaphragm

What does the Diaphragm of the microscope do

Controls the amount of light entering the condenser

What part of the microscope is this

Illuminator

What does the Illuminator do

the light source for the microscope

What part of the microscope is this

Fine focusing knob

What part of the microscope is this

Coarse focusing knob

What part of the microscope is this

Base

What is the magnification of the eyepiece

10x

What is the magnification for scanning power

4x

What is the magnification for low power

10x

What is the magnification for High power

40x

What is the magnification for oil immersion

100x

How to calculate TOTAL magnification

objective magnification times eyepiece magnification

What does parfocal mean

The ability to go from one objective to another with little focusing

What is Resolution/Resolving power (RP)

The ability to distinguish detail clearly and to be able to tell 2 points as separate and not as one

What is Numerical Aperture (always a constant)

The cone of light that enters the lens so we can see fine detail

What two things make up Numerical Aperture

Angular Aperture and Refractive Index

What is Refractive Index

How light travels through a medium

What is Angular Aperture

The angle of light as it goes through the lenses and the filters of the condenser and into the objective

How to calculate Resolution/Resolving power

Wavelength divided by 2x the numerical aperture

What does having a shorter wavelength mean

it means you get a higher resolving power/resolution

What does having a lower resolution (distance) mean

You get a better clarity

Why do blue filters give the best resolution

Because they have the shortest wavelength

Why is having a higher refractive index good

because you get a lower resolution (distance) so you get better clarity

Why do you get better resolution with Oil instead of Air

Because it has a higher Refractive Index

What is the purpose of Immersion oil and how does it affect the image

prevents light from bending as it passes through the slide to the lens, increases resolution and clarity