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41 Terms
What color does the KIA test show if it ferments?
yellow
What color does the KIA test show if it reduces sulfur?
black
What does the butt of the KIA test consist of?
glucose
What does the slant of the KIA test consist of?
lactoe
What type of media is the KIA test?
undefined solid
What is the purpose of the KIA test?
to see if it ferments glucose, lactose, or reduces sulfur
What is the indicator in the KIA test?
phenol red
What are the limitations of the KIA test?
time sensitive, must be innoculated with needle
What is the purpose of the phenol red test?
to determine the ability of an organism to ferment carbohydrates and produce acid.
What type of media is the phenol red test?
undefined liquid
What does the media in the phenol red test contain?
casin peptones, carbohydrates, phenol red, durham tube
What color does the phenol red test show if it ferments?
yellow
What color does the phenol red test show if its neutral?
red
What color does the phenol red test show if it eats peptones?
magenta
What is the limitation of phenol red broth?
time sensitive (48 hrs)
What is the purpose of the oxidase test?
to determine the presence of cytochrome c oxidase in bacteria.
What is the media in the oxidase test?
dry slide, TPP
What are the limitations of the oxidase test?
results must be collected in 20 seconds and no metal innoculating tools
What bacteria contains oxidase?
PSA
What bacteria doesnt contain oxidase?
EC
What is the purpose of the catalase test?
To see if catalase is produced
What does catalase do?
breaks down hydrogen peroxide
What does a positive catalase test show?
bubble formation
What does a negative catalase test show?
no bubble formation
What is the purpose of the nitrate reduction test?
to see if nitrate is converted into nitrite
What is the media of the nitrate reduction test?
undefined liquid
What is the indicator in the nitrate reduction test?
no indicator
What does reagent nitrate A show?
shows nitrite by showing a red color
What does reagent nitrate B show?
confirms nitrite by showing a red color
What does reagent nitrate C show?
indicates nitrate if no color change occurs
What does the oxidation fermentation test do?
see if bacteria ferment and/or oxidizes carbohydrates
What is the media in the oxidation fermentation test
semisolid
What does the media in the oxidation fermentation test contain?
low peptones and high glucose
What is the indicator in the oxidation fermentation test?
Bromothymol blue
What color does bromothymol blue show in neutral pH
green
What color does bromothymol blue show in acidic pH
yellow
What color does bromothymol blue show in basic pH
blue
What is the purpose of the citrate test?
see if bacteria can import and ferment citrate
What are the enzymes in the citrate test?
citrate permease and citratelyase
What is the media in the citrate test?
defined
What does the media in the citrate test contain?
citrate, ammonia, and bromothymol blue