TOOBES

What is plasma

liquid portion of unclotted blood that still contains clotting factors

What is serum

liquid portion of blood that has been allowed to coagulate so clotting factors are no longer present

What is the order of draw?

1. Blood culture bottles

2. Discard tubes

3. Light blue top

4. Red top

5. Gold/Tiger/Marble top

6. Light green top

7. Dark green top

8. Pink top

9. Lavender top

10. Gray top

11. Dark blue

Why is incorrect order of draw bad?

can result in cross contamination

Blood Cultures

1. what additive?

2. what lab responsible for testing?

3. inversions?

4. common test

Must be done sterile and 3

1. nutrient broth (SPS - )

2. microbiology

3. none

PT (prothrombin time) - evaluates the extrinsic system of coagulation casace

PTT (partial thromboplastin time) - evaluates intrinsic system of coagulation cascade

aPTT (activated PTT) - evaluates how long the patient’s blood take to form a clot

Light blue tube top

1. what additive?

2. yields?

3. what lab responsible for testing?

4. inversions?

5. common test

1. Sodium citrate (anticoagulant)

2. plasma

3. coagulation

4. 3-4

5. PT, PTT, aPTT

Clear tube top

discard tube

Red tube top

1. what additive?

2. yields?

3. what lab responsible for testing?

4. inversions?

1. none (glass) or clot activator (plastic)

2. serum

3. chemistry

4. 0

Tiger top/Marble top/Red-gray speckled top/Gold top tubes

1. what additive?

2. yields?

3. what lab responsible for testing?

4. inversions?

5. common test

1. silica (clot activator) and thixotropic gel (serum separator)

2. serum

3. chemistry

4. 8

BMP - basic metabolic panel

CMP - complete/comprehensive metabolic panel

Serum pregnancy test

Lipids and cholesterol

Light green top tube

1. what additive?

2. yields?

3. what lab responsible for testing?

4. inversions?

5. common test

1. Lithium heparin (anticoagulant) and thixotropic gel (plasma separator)

2. plasma (also called Plasma Separation Tube)

3. chemistry

4. 8

STAT (electrolytes)

hCG

hCG qualitative

hCG quantitative

Dark green tube top

1. what additive?

2. yields?

3. what lab responsible for testing?

4. inversions?

5. common test

1. sodium heparin (anticoagulant)

2. plasma

3. Chemistry

4. 8

5. ammonia (must place in ice)

Pink tube top

1. what additive?

2. yields?

3. what lab responsible for testing?

4. inversions?

5. common test

1. Potassium EDTA

2. plasma

3. blood bank

4. 8

TSR (type and screen ratio)

Cross match

Lavender or Light/Dark purple tube top

1. what additive?

2. yields?

3. what lab responsible for testing?

4. inversions?

5. common test

1. EDTA

2. plasma

3. hematology

4. 8

ESR (erythrocyte sedimentation rate)

sickle cell

CBC (complete blood count)

Hematocrit

Platelets

Gray tube top

1. what additive?

2. yields?

3. what lab responsible for testing?

4. inversions?

5. common test

1. sodium fluoride (antiglycolytic agent) and potassium oxalate (anticoagulant)

2. plasma

3. chemistry

4. 8

OGTT (oral glucose tolerance test)

Lactic acid

alcohol levels (dont use alcohol wipes for cleaning site bc can cause false positive)

Royal blue tube top

1. what additive?

2. yields?

3. what lab responsible for testing?

4. inversions?

5. common test

1. none (red stripe) or EDTA (blue/lavender stripe)

2. serum (red stripe) or plasma (blue/lavender stripe)

3. toxicology

4. 8

5. lead poisoning and heavy metal toxicology