pdh complex

Where does glycolysis occur?

cytoplasm

What product is made from glycolysis?p

pyruvate

Pyruvate acts as a substrate for what?

PDH complex

where does gluconeogenesis begin/mostly occur in?

matrix

where is the PDH complex?

matrix

what is the main goal of the PDH complex?

convert pyruvate into acetyl CoA and oxidize the lipoyl group back to its original state

steps for conversion of pyruvate to acetyl COA by the PDH complex?

1) decarboxylation 2) oxidation 3) transfer to CoA

PDH contains what enzymes?

E1, E2, E3

E1 is associated with which catalytic coenzyme?

TPP

E2 is associated with which catalytic coenzyme?

lipoamide

E3 is associated with which catalytic coenzyme?

FAD

What are the three catalytic coenzymes?

TPP, lipoamide, FAD

are catalytic coenzymes consumed in the reaction?

no

what are the two stoichiometric coenzymes?

CoA, NAD+

what are stoichiometric coenzymes used for?

substrates

IF the pdh complex is inhibited, glycolysis will be (affected/unaffected). Pyruvate with (increase/decrease)

unaffected, increase

If the pdh complex is inhibited, pyruvate (can/cannot) be converted into acetyl-CoA. Acetyl-Coa will (increase/decrease).

cannot, decrease

During low energy charge, will PDH activity increase or decrease?

increase

During the phosphorylation of PDH, will PDH activity increase or decrease?

decrease

when levels of acetyl coa are high, will PDH activity increase or decrease?

decrease

when levels of NADH are high, will PDH activity increase or decrease

decrease

What is the binding site for PDH when energy charge is low?

PDH kinase

what is the binding site for the phosphorylation of PDH when PDH activity is inhibited?

E1

what is the binding site for high levels of acetyl CoA and decreased PDH activity?

E2

What is the binding site for high levels of NADH and decreased PDH activity?

E3

How many turns of TCA cycle are there per glucose?

2

what is the first step of TCA cycle?

acetyl group of acetyl CoA joins the cycle by combining with oxaloacetate and forms citrate

TCA cycle is amphibolic, meaning

it participates in both catabolic and anabolic processes.

oxaloacetate to gluconeogenesis is (high/low) energy charge

high

oxaloacetate to citrate is (high/low) energy charge

low

what are the regulatory steps in TCA cycle?

the most negative delta g prime steps: 1,3, 4

steps 1, 3, and 4 in TCA cycle are _ enzymes

allosteric