Botany Lab - Module 1-2: Microscope & Magnification

Proper care and maintenance of a microscope is of paramount importance and can help extend its life.

_____________________________ of a microscope is of paramount importance and can help extend its life. (Edu-Lab Scientific Resources, Feb 2017)

hold it by the base and the metal support arm.

How to hold a microscope

keep your microscope covered when not in use

Proper storage of microscope

Oil immersion

technique used to increase there solving power of a microscope; Both the objective lens and sample are immersed in a transparent oil of high refractive index so that high magnifications can be achieved while still maintaining good resolution

turn off the illuminator and wait for it to cool down before putting it away.

How to take care of bulbs

Rotate the nosepiece of the microscope all the way down to its lowest level when you have finished using the microscope

What to do after using microscope?

Microscopium

an instrument for viewing what is small

Skopion

means of viewing

Skopien

look at

Eyepiece lens

contains the ocular lens; magnification ranging from 5X to 30X

10X or 15X

Most common magnification used

Eyepiece tube

connects the eyepiece and ocular lens to the objective lens

Microscope Arm

connects the eyepiece tube to the base

Base

provides stability and support; holds the illumnitor

Stage and Stage clips

platform for slides that hold the specimen

Nosepiece

contains the objective lens

Objective lens

lenses that protrude downward over the specimen

4x

Scanning lens magnification

10x

LPO magnification

40x

HPO Magnification

100x

Oil immersion magnification

Rack stop

Prevents users from moving the objectives too close to the side

Control Focus knobs

Turning the knobs adjusts the distance betweenthe stage and the lens

coarse adjustment knob

used to bring the specimen into initial focus -- visible butnot sharp

fine adjustment knob

turned tobring the specimen into sharp focus

Condenser

concentrates light

diaphragm

regulate entry of light

Dry Mount

Mounting only the specimen

Wet mount

a drop of water is placed on the dry mounted sample

Staining mount

a coloring liquid is added to sample until it is fully covered

Power of resolution

measure the ability to tell 2 points apart

Magnification of a microscope

product of objective and ocular magnification

Numerical aperture

represents a performance number that can be compared to the relative aperture (f-number) of a camera lens; The larger the N.A., the higher the resolving power is.

Working Distance

refers to the distance from the cover glass to the nearest point of the objective

Focal depth

refers to the distance between the upper and lower limits of sharpness in the image formed by an optical system; The larger the N.A. of an objective the shallower the focal depth is

Field number

This is a number that represents the diameter in mm of the image of the field diaphragm that is formed by the lens in front of it

Field of view diameter

The actual size of the field of view in mm on the object surface