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Capsule staining and endospore staining
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In capsule staining, the background is primarily stained by what chemical(s)?
Nigrosin
What chemical(s) will stain the cell?
Crystal Violet
What isn’t stained after performing the capsule stain?
The capsule
Which statement is true?
Capsules are made of sugar
What would happen if nigrosin wasn’t added to the slide?
The background wouldn’t stain purple
What would happen if the nigrosin didn’t completely dry before washing with Crystal Violet?
The background won’t stain purple
What would happen if Crystal Violet wasn’t added to slide?
The cells won’t be stained purple
What would happen if you heatfix the slide more than once?
The capsule will burn
Klebsiella Pneumenaie was observed to have a white halo around its cell membrane. What conclusions can be made?
Klebsiella Pneumenaie has a capsule around it