1. observe both normal and sick organisms, take samples from each
2. isolate microbes from one another and characterize the microbes found in the sick organism
3. inject normal organisms with the microbes and observe
4. if an organism displays the same symptoms/signs of the initial disease take samples
5. isolate microbes from one another and characterize the microbes
6. compare the initial isolates with the microbe found in 5, if they are the same, then you have found the cause