Part 7: Gram positive

Bacillus

Gram (+) bacilli; Spore former; aerobic

B. anthracis

Woolsorter’s disease

B. cereus

Catalase (+)

Lecithinase (+)

Motility (+)

BAP: Beta

C. perfringens

The only non-motile Clostridium

C. perfringens

The only lecithinase (+) Clostridium

C. botulinum

The only lipase positive Clostridium

C. perfringens

The only lactose positive Costridium

C. tetani

The only glucose negative C. tetani

brown halo

A _____________ surrounding the colony, owing to cystinase activity, is useful in differentiating feature of C. diptheriae, C. ulcerans, C. pseudotuebculosis

C. diptheriae intermedius type

Produces small colonies (0.5 mm) on Blood Agar, black colonies with gray borders on tellurite medium

Erysipherothrix rhusiopathiae

Agent of eryspeloid cutaneous, inflammation of the hand or fingers (seal finger or whale finger)

RIPE

Rifampin

Isoniazid

Pyrazinamide

Ethambutol

1st phase Antibiotics of M. tubercolosis

RI

2nd phase antibiotics of M. tuberculosis

N-acetyl - L - cysteine

NALC

NALC

Digesting agent for Mycobacteria

NaOH

Decontaminating agent for Mycobacteria

+/- Repeated on second slide

CDC method to report AFB: 0

1-9/300 fields

CDC method to report AFB: 1+

1-9/100 fields

CDC method to report AFB : 2+

1-9/ fields

CDC method to report AFB: 3+

>9/ fields

CDC method to report AFB: 4+

Mycobacterium leprae

Foamy appearance on tissue juice

Footpad of Mice/ Armadillo

M. leprae is cultured on

M. bovis

TB in animals

M. avium

TB in birds

“May isang MARINUM sa ASIAcum, SIMIar sa KANSAs”

M. marinum

M. asiaticum

M. simiae

M. kansasii

Group 1/ Photochromogens in Runyoun Classification

HAM-T

M. haemophilum

M. avium-intracellulare complex

M. malmoense

M. terrae-triviale

Group 3/ Nonphotochromogens in Runyoun Classification

“Mem Play (Phlei) the Fortnight (Fortuit)

M. smegmatis

M. phlei

M. fortuitum-chenolei complex

Group 4/ Rapid Growers in Runyoun Classification

Actonomycetes

Agent of Lumpy jaw

Nocardia

Gram positive, partially acid fast, aerobes

Spirillum minus

Causes ratbite fever in humans and is referred as SODOKO

Borrelia recurrentis

Agent of louse-borne relapsing fever

Borrelia burgdorferi

Agent of Lyme disease

Borrelia burgdorferi

#1 Cause of tickborne disease in US

Stage 1

Borrelia burgdorferi Stage: Appearance of lesion known as erythema chronicum migrans

Stage 2

Borrelia burgdorferi Stage: Dissemination through blood

Stage 3

Borrelia burgdorferi Stage: Neurological abnormalitis, arthritis and skin lesions

B. parkeri/ B. harmsii

Agent of Tick-Borne Relapsing fever

Hatchinson’s triad for Syphilis

What triad?

1. Keratitis

2. Notched teeth

   1. Deafness

3 days @ 2-8 degC

T. pallidum dies in _____ days

Primary

Appearance of hard chancre

Secondary

Appearance of condylomata lata

Tertiary

Appearance of gummas

Yaws

Non-venereal disease; ulceration that can be found on skin and mouth

Bejel

Lesions in oral cavity, oral mucosa, skin, bones and nasopharynx by subs endemicum

Pinta

Ulcerative skin disease by Treponema careteum

Vincent’s disease

Destructive lesions of the gums

Weil’s disease

Severe form of leptospirosis

Ellinghauses-McCullough-Johnson-Harris and Fetcher’s

Culture media of Leptospira