Factors effecting enzyme activity

Q1: What happens to enzymes at high temperatures?

A: They denature (lose functional shape).

Q2: What is the optimum temperature for enzymes?

A: The temperature at which they work fastest before denaturation.

Q3: How does concentration affect enzyme activity?

A: More substrate → more collisions → higher rate until saturation.

Q4: What happens when all active sites are used?

A: The reaction rate plateaus (no further increase).

Q5: How does pH affect enzymes?

A: Too high/low pH can denature enzymes or reduce binding efficiency.

Q6: What is the optimum pH?

A: The pH level at which the enzyme works best.

Q7: How does surface area influence rate?

A: More surface = more exposed particles = more collisions.

Q8: What causes enzymes to denature?

A: Extreme temperatures or incorrect pH.

Q9: What is the relationship between temperature and kinetic energy?

A: Higher temp = more kinetic energy = more frequent collisions.

Q10: Why does rate decrease after optimum temperature?

A: Enzyme denaturation reduces active site function.